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Fleck D, Kenzler L, Mundt N, et al. ATP activation of peritubular cells drives testicular sperm transport. Elife. 2021;10doi: 10.7554/eLife.62885.
Fleck, D., Kenzler, L., Mundt, N., Strauch, M., Uesaka, N., Moosmann, R., Bruentgens, F., Missel, A., Mayerhofer, A., Merhof, D., Spehr, J., & Spehr, M. (2021). ATP activation of peritubular cells drives testicular sperm transport. eLife, 10. https://doi.org/10.7554/eLife.62885
Fleck, David, et al. "ATP activation of peritubular cells drives testicular sperm transport." eLife vol. 10 (2021). doi: https://doi.org/10.7554/eLife.62885
Fleck D, Kenzler L, Mundt N, Strauch M, Uesaka N, Moosmann R, Bruentgens F, Missel A, Mayerhofer A, Merhof D, Spehr J, Spehr M. ATP activation of peritubular cells drives testicular sperm transport. Elife. 2021 Jan 27;10. doi: 10.7554/eLife.62885. PMID: 33502316; PMCID: PMC7840184.
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Elife. 2021 Jan 27;10. doi: 10.7554/eLife.62885.

ATP activation of peritubular cells drives testicular sperm transport.

eLife

David Fleck, Lina Kenzler, Nadine Mundt, Martin Strauch, Naofumi Uesaka, Robert Moosmann, Felicitas Bruentgens, Annika Missel, Artur Mayerhofer, Dorit Merhof, Jennifer Spehr, Marc Spehr

Affiliations

  1. Department of Chemosensation, Institute for Biology II, RWTH Aachen University, Aachen, Germany.
  2. Research Training Group 2416 MultiSenses - MultiScales, RWTH Aachen University, Aachen, Germany.
  3. Institute of Imaging and Computer Vision, RWTH Aachen University, Aachen, Germany.
  4. Department of Cognitive Neurobiology, Tokyo Medical and Dental University, Tokyo, Japan.
  5. Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.

PMID: 33502316 PMCID: PMC7840184 DOI: 10.7554/eLife.62885

Abstract

Spermatogenesis, the complex process of male germ cell proliferation, differentiation, and maturation, is the basis of male fertility. In the seminiferous tubules of the testes, spermatozoa are constantly generated from spermatogonial stem cells through a stereotyped sequence of mitotic and meiotic divisions. The basic physiological principles, however, that control both maturation and luminal transport of the still immotile spermatozoa within the seminiferous tubules remain poorly, if at all, defined. Here, we show that coordinated contractions of smooth muscle-like testicular peritubular cells provide the propulsive force for luminal sperm transport toward the rete testis. Using a mouse model for in vivo imaging, we describe and quantify spontaneous tubular contractions and show a causal relationship between peritubular Ca

© 2021, Fleck et al.

Keywords: P2 receptors; cell biology; germ cell development; human; male reproduction; mouse; purinergic signaling; spermatogenesis

Conflict of interest statement

DF, LK, NM, MS, NU, RM, FB, AM, AM, DM, JS, MS No competing interests declared

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