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Duan C, Jimenez JM, Goergen C, et al. Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics. J Voice. 2021;doi: 10.1016/j.jvoice.2021.01.014.
Duan, C., Jimenez, J. M., Goergen, C., Cox, A., Sivasankar, P. M., & Calve, S. (2021). Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics. Journal of voice : official journal of the Voice Foundation, . https://doi.org/10.1016/j.jvoice.2021.01.014
Duan, Chenwei, et al. "Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics." Journal of voice : official journal of the Voice Foundation vol. (2021). doi: https://doi.org/10.1016/j.jvoice.2021.01.014
Duan C, Jimenez JM, Goergen C, Cox A, Sivasankar PM, Calve S. Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics. J Voice. 2021 Feb 01; doi: 10.1016/j.jvoice.2021.01.014. Epub 2021 Feb 01. PMID: 33541766; PMCID: PMC8325720.
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J Voice. 2021 Feb 01; doi: 10.1016/j.jvoice.2021.01.014. Epub 2021 Feb 01.

Hydration State and Hyaluronidase Treatment Significantly Affect Porcine Vocal Fold Biomechanics.

Journal of voice : official journal of the Voice Foundation

Chenwei Duan, Julian M Jimenez, Craig Goergen, Abigail Cox, Preeti M Sivasankar, Sarah Calve

Affiliations

  1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana; Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana.
  2. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana; Department of Mechanical Engineering, University of Colorado-Boulder, Boulder, Colorado.
  3. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana.
  4. Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana.
  5. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana; Department of Mechanical Engineering, University of Colorado-Boulder, Boulder, Colorado. Electronic address: [email protected].

PMID: 33541766 PMCID: PMC8325720 DOI: 10.1016/j.jvoice.2021.01.014

Abstract

OBJECTIVES: The understanding of vocal fold hydration state, including dehydrated, euhydrated, rehydrated tissue, and how hydration affects vocal fold biomechanical properties is still evolving. Although clinical observations support the benefits of increasing vocal fold hydration after dehydrating events, more mechanistic information on the effects of vocal fold dehydration and the beneficial effects of rehydration are needed. Alterations to hyaluronic acid (HA), an important component of the vocal fold extracellular matrix, are likely to influence the biomechanical properties of vocal folds. In this study, we investigated the influence of hydration state and HA on vocal fold tissue stiffness via biomechanical testing.

STUDY DESIGN: Prospective, ex vivo study design.

METHODS: Fresh porcine vocal folds (N = 18) were examined following sequential immersion in hypertonic (dehydration) and isotonic solutions (rehydration). In a separate experiment, vocal folds were incubated in hyaluronidase (Hyal) to remove HA. Control tissues were not exposed to any challenges. A custom micromechanical system with a microforce sensing probe was used to measure the force-displacement response. Optical strain was calculated, and ultrasound imaging was used to measure tissue cross-sectional area to obtain stress-strain curves.

RESULTS: Significant increases (P ≤ 0.05) were found in tangent moduli between dehydrated and rehydrated vocal folds at strains of ε = 0.15. The tangent moduli of Hyal-digested tissues significantly increased at both ε = 0.15 and 0.3 (P ≤ 0.05).

CONCLUSION: Vocal fold dehydration increased tissue stiffness and rehydration reduced the stiffness. Loss of HA increased vocal fold stiffness, suggesting a potential mechanical role for HA in euhydrated vocal folds.

Copyright © 2021 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Keywords: Biomechanics; Dehydration; Hyaluronic acid; Rehydration; Vocal folds

References

  1. J Speech Hear Res. 1994 Oct;37(5):1001-7 - PubMed
  2. J Voice. 2011 Mar;25(2):249-53 - PubMed
  3. J Acoust Soc Am. 2008 Jun;123(6):4572-81 - PubMed
  4. J Acoust Soc Am. 2008 Mar;123(3):1627-36 - PubMed
  5. J Voice. 2013 Nov;27(6):659-67 - PubMed
  6. J Speech Hear Res. 1984 Jun;27(2):212-9 - PubMed
  7. Nat Methods. 2012 Jun 28;9(7):676-82 - PubMed
  8. Ann Otol Rhinol Laryngol. 2001 Dec;110(12):1120-5 - PubMed
  9. J Voice. 2020 Jan;34(1):1-8 - PubMed
  10. Laryngoscope. 2021 Apr;131(4):839-845 - PubMed
  11. J Voice. 1994 Mar;8(1):30-47 - PubMed
  12. J Appl Physiol (1985). 2006 Oct;101(4):1113-7 - PubMed
  13. J Acoust Soc Am. 2016 Apr;139(4):1493 - PubMed
  14. Curr Opin Struct Biol. 2003 Oct;13(5):612-20 - PubMed
  15. J Acoust Soc Am. 2017 Oct;142(4):2311 - PubMed
  16. J Acoust Soc Am. 2016 Oct;140(4):2614 - PubMed
  17. J Voice. 2010 Sep;24(5):626-35 - PubMed
  18. J Voice. 2002 Sep;16(3):303-9 - PubMed
  19. Curr Opin Otolaryngol Head Neck Surg. 2010 Dec;18(6):498-502 - PubMed
  20. J Voice. 2012 Nov;26(6):706-10 - PubMed
  21. J Appl Physiol (1985). 2005 Mar;98(3):1006-12 - PubMed
  22. Biomed Mater Eng. 2004;14(1):13-22 - PubMed
  23. Biomatter. 2013 Jan-Mar;3(1): - PubMed
  24. J Voice. 2018 Jul;32(4):492-498 - PubMed
  25. J Speech Lang Hear Res. 2002 Apr;45(2):268-81 - PubMed
  26. J Voice. 2009 Jan;23(1):40-50 - PubMed
  27. Ann Otol Rhinol Laryngol. 2009 Feb;118(2):154-9 - PubMed
  28. J Acoust Soc Am. 2012 Sep;132(3):1626-35 - PubMed
  29. J Voice. 2009 Nov;23(6):658-65 - PubMed
  30. Mater Sci Eng C Mater Biol Appl. 2016 Jul 1;64:444-453 - PubMed
  31. J Voice. 2011 Mar;25(2):130-6 - PubMed
  32. J Voice. 2019 Jan;33(1):125.e13-125.e28 - PubMed
  33. Laryngoscope. 2000 May;110(5 Pt 1):814-24 - PubMed
  34. J Voice. 2017 Jan;31(1):111.e29-111.e36 - PubMed
  35. J Voice. 2014 Jul;28(4):406-10 - PubMed
  36. Otolaryngol Head Neck Surg. 2011 Jan;144(1):108-13 - PubMed
  37. J Voice. 2021 Jan;35(1):77-84 - PubMed
  38. J Biol Chem. 2018 Jan 12;293(2):567-578 - PubMed
  39. Sci Rep. 2016 Apr 12;6:24272 - PubMed
  40. Otolaryngol Head Neck Surg. 2001 Jun;124(6):607-14 - PubMed

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