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Yousaf R, Khan I, Shehzad W, et al. Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan. Infect Dis Rep. 2021;13(1):166-172doi: 10.3390/idr13010018.
Yousaf, R., Khan, I., Shehzad, W., Hussain, R., Ali, S., Neubauer, H., & Wareth, G. (2021). Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan. Infectious disease reports, 13(1), 166-172. https://doi.org/10.3390/idr13010018
Yousaf, Riffat, et al. "Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan." Infectious disease reports vol. 13,1 (2021): 166-172. doi: https://doi.org/10.3390/idr13010018
Yousaf R, Khan I, Shehzad W, Hussain R, Ali S, Neubauer H, Wareth G. Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan. Infect Dis Rep. 2021 Feb 08;13(1):166-172. doi: 10.3390/idr13010018. PMID: 33567632; PMCID: PMC7931085.
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Infect Dis Rep. 2021 Feb 08;13(1):166-172. doi: 10.3390/idr13010018.

Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan.

Infectious disease reports

Riffat Yousaf, Iahtasham Khan, Wasim Shehzad, Riaz Hussain, Shahzad Ali, Heinrich Neubauer, Gamal Wareth

Affiliations

  1. Institute of Biochemistry & Biotechnology, University of Veterinary and Animal Sciences Lahore, Lahore 54000, Pakistan.
  2. Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore Sub-Campus Jhang, Lahore 35200, Pakistan.
  3. Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
  4. Wildlife Epidemiology and Molecular Microbiology Laboratory (One Health Research Group), Discipline of Zoology, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore 35200, Pakistan.
  5. Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.

PMID: 33567632 PMCID: PMC7931085 DOI: 10.3390/idr13010018

Abstract

Brucellosis is one of the most notorious zoonoses worldwide. The disease is common and endemic in humans and animals of Pakistan, but lack of awareness and lack of research have resulted in an increased incidence in the human population. The present study aimed to determine the seroprevalence and at molecular detection of brucellosis in patients with clinical symptoms in six different hospitals from Lahore, which is the capital city of Punjab province. A total of 218 blood samples were collected from hospitalized patients. The samples were initially screened by the Rose Bengal Plate Test (RBPT), and then quantitative real-time PCR (qRT-PCR) was applied. An overall seroprevalence of 17% (37/218) was found. The highest prevalence was found at the Lady Health center (36.53%), which was followed by the Lady Willingdon Hospital (28.6%). Female patients showed a higher seroprevalence than males and peaked at 34% (n = 32) for women who suffered from abortion. In total, 16.8% of patients younger than 30 years showed seropositive reactions, while the prevalence was 19% in patients between 31 and 50. Thirty-three DNA samples from 24 seropositive and nine seronegative patients tested positive, 32 samples were found positive for

Keywords: Lahore; RBPT; RT-PCR; brucellosis; hospital patients

References

  1. Vet Med Sci. 2019 Aug;5(3):390-401 - PubMed
  2. Monogr Ser World Health Organ. 1975;(55):1-163 - PubMed
  3. J Infect Public Health. 2020 May 18;: - PubMed
  4. Foodborne Pathog Dis. 2012 Mar;9(3):190-7 - PubMed
  5. J Travel Med. 2004 Jan-Feb;11(1):49-55 - PubMed
  6. Int J Environ Res Public Health. 2020 Sep 21;17(18): - PubMed
  7. Vet Sci. 2018 Mar 09;5(1): - PubMed
  8. PLoS Negl Trop Dis. 2014 Jul 24;8(7):e3008 - PubMed
  9. J Res Med Sci. 2011 Oct;16(10):1366-71 - PubMed
  10. Front Vet Sci. 2020 Sep 02;7:526 - PubMed
  11. J Clin Microbiol. 2004 Mar;42(3):1290-3 - PubMed
  12. Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):194-9 - PubMed
  13. BMC Infect Dis. 2016 Sep 02;16:468 - PubMed
  14. J Pak Med Assoc. 2020 Sep;70(9):1625-1626 - PubMed
  15. J Ayub Med Coll Abbottabad. 2018 Oct-Dec;30(4):566-570 - PubMed
  16. Zoonoses Public Health. 2009 May;56(4):183-7 - PubMed
  17. Foodborne Pathog Dis. 2013 Jun;10(6):500-5 - PubMed
  18. Emerg Infect Dis. 1997 Apr-Jun;3(2):213-21 - PubMed
  19. J Pak Med Assoc. 2010 Dec;60(12):1031-4 - PubMed
  20. Lancet Infect Dis. 2014 Jun;14(6):520-6 - PubMed
  21. Bull World Health Organ. 2003;81(12):867-76 - PubMed
  22. Int J Environ Res Public Health. 2020 Feb 21;17(4): - PubMed
  23. PLoS One. 2017 Mar 16;12(3):e0173365 - PubMed
  24. ScientificWorldJournal. 2012;2012:104239 - PubMed
  25. Recent Pat Antiinfect Drug Discov. 2013 Apr;8(1):34-41 - PubMed
  26. Indian J Med Microbiol. 2007 Jan;25(1):28-31 - PubMed
  27. Int J Environ Res Public Health. 2019 Oct 23;16(21): - PubMed
  28. Pathogens. 2020 Aug 19;9(9): - PubMed

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