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de Souza VG, Bandeira LB, Souza NCSE, et al. Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (. J Dev Orig Health Dis. 2021;1-12doi: 10.1017/S2040174421000040.
de Souza, V. G., Bandeira, L. B., Souza, N. C. S. E., Taboga, S. R., Martins, T. M. M., & Silva Perez, A. P. (2021). Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (. Journal of developmental origins of health and disease, 1-12. https://doi.org/10.1017/S2040174421000040
de Souza, Vinícius Gonçalves, et al. "Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (." Journal of developmental origins of health and disease vol. (2021): 1-12. doi: https://doi.org/10.1017/S2040174421000040
de Souza VG, Bandeira LB, Souza NCSE, Taboga SR, Martins TMM, Silva Perez AP. Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (. J Dev Orig Health Dis. 2021 Mar 02;1-12. doi: 10.1017/S2040174421000040. Epub 2021 Mar 02. PMID: 33650479.
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J Dev Orig Health Dis. 2021 Mar 02;1-12. doi: 10.1017/S2040174421000040. Epub 2021 Mar 02.

Prenatal and pubertal exposure to 17α-ethinylestradiol disrupts folliculogenesis and promotes morphophysiological changes in ovaries of old gerbils (.

Journal of developmental origins of health and disease

Vinícius Gonçalves de Souza, Laura Borges Bandeira, Nátaly Caroline Silva E Souza, Sebastião Roberto Taboga, Tracy Martina Marques Martins, Ana Paula Silva Perez

Affiliations

  1. Institute of Health Sciences, Medicine Course, Federal University of Jataí, UFJ, Jataí, Goiás75801-615, Brazil.
  2. Department of Biology, Laboratory of Microscopy and Microanalysis, São Paulo State University - UNESP, São José do Rio Preto, São Paulo15054-000, Brazil.
  3. Department of Structural and Functional Biology, State University of Campinas - UNICAMP, Campinas, São Paulo13083-970, Brazil.
  4. Institute of Health Sciences, Medicine Course and Graduate Program of Animal Bioscience, Federal University of Jataí, UFJ, Jataí, Goiás75801-615, Brazil.

PMID: 33650479 DOI: 10.1017/S2040174421000040

Abstract

17α-Ethinylestradiol is an endocrine-disrupting chemical that make up most contraceptive pills and can be found in the environment. Exposure to ethinylestradiol in different development periods may promote changes in morphophysiological parameters of reproductive and endocrine organs. Considering that the effects of low doses (15 µg/kg/day) of ethinylestradiol in ovaries from 12-month-old female gerbils (Meriones unguiculatus) were investigated. Four experimental groups used were control (without treatment), EE/PRE (treated from the 18th to the 22nd gestational day), EE/PUB (treated from the 42nd to the 49th day of life), and EE/PRE-PUB (treated in the both periods). The animals were euthanized at 12 months. Testosterone and 17β-estradiol levels were measured. The ovaries were stained with Hematoxylin and Eosin, Periodic Acid Schiff, and Gomori's Trichome. The follicles, corpus luteum, interstitial gland, lipofuscin, ovarian epithelium, and tunica albuginea were analyzed. Estradiol was higher in EE/PRE and EE/PUB groups, while testosterone was higher only in EE/PUB group. The main changes in follicle count occurred in EE/PUB and EE/PRE-PUB groups, with higher primordial follicle count and lower maturation of follicles. The corpus luteum was more evident in EE/PRE group. No differences were found in atretic follicles count. A higher area occupied by interstitial gland cells and lipofuscin deposit in these cells was noted in EE/PUB and EE/PRE-PUB groups. Higher epithelium height and thicker tunic albuginea were showed in treated groups. These results suggest that exposure to doses of EE2 in prenatal and pubertal periods of the development leads to morphological changes in senile ovaries.

Keywords: Gerbillinae; contraceptive agents; endocrine disruptors; ovary

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