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Koo CH, Baik J, Shin HJ, et al. Neurotoxic Effects of Local Anesthetics on Developing Motor Neurons in a Rat Model. J Clin Med. 2021;10(5)doi: 10.3390/jcm10050901.
Koo, C. H., Baik, J., Shin, H. J., Kim, J. H., Ryu, J. H., & Han, S. H. (2021). Neurotoxic Effects of Local Anesthetics on Developing Motor Neurons in a Rat Model. Journal of clinical medicine, 10(5), . https://doi.org/10.3390/jcm10050901
Koo, Chang-Hoon, et al. "Neurotoxic Effects of Local Anesthetics on Developing Motor Neurons in a Rat Model." Journal of clinical medicine vol. 10,5 (2021). doi: https://doi.org/10.3390/jcm10050901
Koo CH, Baik J, Shin HJ, Kim JH, Ryu JH, Han SH. Neurotoxic Effects of Local Anesthetics on Developing Motor Neurons in a Rat Model. J Clin Med. 2021 Feb 25;10(5). doi: 10.3390/jcm10050901. PMID: 33668828; PMCID: PMC7956179.
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J Clin Med. 2021 Feb 25;10(5). doi: 10.3390/jcm10050901.

Neurotoxic Effects of Local Anesthetics on Developing Motor Neurons in a Rat Model.

Journal of clinical medicine

Chang-Hoon Koo, Jiseok Baik, Hyun-Jung Shin, Jin-Hee Kim, Jung-Hee Ryu, Sung-Hee Han

Affiliations

  1. Department of Anesthesiology & Pain Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Korea.
  2. Department of Anesthesia & Pain Medicine, Pusan National University Hospital, Busan 49241, Korea.
  3. Department of Anesthesiology & Pain Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.

PMID: 33668828 PMCID: PMC7956179 DOI: 10.3390/jcm10050901

Abstract

Neurotoxic effects of local anesthetics (LAs) on developing motor neurons have not been documented. We investigated the neurotoxic effects of LAs on developing motor neurons in terms of cell viability, cytotoxicity, reactive oxygen species (ROS), and apoptosis. Embryonic spinal cord motor neurons were isolated from Sprague-Dawley rat fetuses and exposed to one of the three LAs-lidocaine, bupivacaine, or ropivacaine-at concentrations of 1, 10, 100, or 1000 µM. The exposure duration was set to 1 or 24 h. The neurotoxic effects of LAs were determined by evaluating the following: cell viability, cytotoxicity, ROS production, and apoptosis. In the 1-h exposure group, the motor neurons exposed to lidocaine and bupivacaine had reduced cell viability and increased cytotoxicity, ROS, and apoptosis in a concentration-dependent manner. Lidocaine showed the highest toxicity, followed by bupivacaine. In the 24-h exposure group, all three LAs showed significant effects (decreased cell viability and increased cytotoxicity, ROS, and apoptosis) on the motor neurons in a concentration-dependent manner. The neurotoxic effects of lidocaine were greater than those of bupivacaine and ropivacaine. Ropivacaine appeared to have the least effect on motor neurons. This study identified the neurotoxic effects of lidocaine and bupivacaine on developing spinal cord motor neurons.

Keywords: apoptosis; bupivacaine; lidocaine; motor neurons; ropivacaine; toxicity

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