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Tan SL, Alibhai D, Cross SJ, et al. Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy. Bio Protoc. 2020;10(21):e3806doi: 10.21769/BioProtoc.3806.
Tan, S. L., Alibhai, D., Cross, S. J., Thompson, H., & Wülfing, C. (2020). Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy. Bio-protocol, 10(21), e3806. https://doi.org/10.21769/BioProtoc.3806
Tan, Sin Lih, et al. "Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy." Bio-protocol vol. 10,21 (2020): e3806. doi: https://doi.org/10.21769/BioProtoc.3806
Tan SL, Alibhai D, Cross SJ, Thompson H, Wülfing C. Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy. Bio Protoc. 2020 Nov 05;10(21):e3806. doi: 10.21769/BioProtoc.3806. eCollection 2020 Nov 05. PMID: 33659460; PMCID: PMC7842649.
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Bio Protoc. 2020 Nov 05;10(21):e3806. doi: 10.21769/BioProtoc.3806. eCollection 2020 Nov 05.

Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy.

Bio-protocol

Sin Lih Tan, Dominic Alibhai, Stephen J Cross, Harry Thompson, Christoph Wülfing

Affiliations

  1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom.
  2. Wolfson BioImaging Facility, University of Bristol, Bristol, United Kingdom.
  3. School of Biochemistry, University of Bristol, Bristol, United Kingdom.

PMID: 33659460 PMCID: PMC7842649 DOI: 10.21769/BioProtoc.3806

Abstract

Supramolecular signaling assemblies are of interest for their unique signaling properties. A µm scale signaling assembly, the central supramolecular signaling cluster (cSMAC), forms at the center interface of T cells activated by antigen presenting cells (APC). The adaptor protein linker for activation of T cells (LAT) is a key cSMAC component. The cSMAC has widely been studied using total internal reflection fluorescence microscopy of CD4

Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Immune Synapse; LAT; STED; T cell activation; cSMAC

Conflict of interest statement

Competing interestsThe authors declare no competing interests.

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