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Bio Protoc. 2020 Mar 05;10(5):e3535. doi: 10.21769/BioProtoc.3535. eCollection 2020 Mar 05.

Tandem Tag Assay Optimized for Semi-automated .

Bio-protocol

Adrian N Dauphinee, Jonas A Ohlsson, Elena A Minina

Affiliations

  1. Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, P.O. Box 7015, Uppsala, SE-750 07, Sweden.
  2. COS, Heidelberg University. Im Neuenheimer Feld 230. 69120 Heidelberg, Germany.

PMID: 33659509 PMCID: PMC7842398 DOI: 10.21769/BioProtoc.3535

Abstract

Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis.

Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Autophagic activity in vivo; Arabidopsis; Autophagic activity; Autophagic flux; High-throughput autophagic activity measurement; In planta autophagic activity assay ; Plant autophagy; Tandem tag

Conflict of interest statement

Competing interestsThe authors declare no competing financial interests.

References

  1. Autophagy. 2012 Aug;8(8):1215-26 - PubMed
  2. Autophagy. 2016;12(1):1-222 - PubMed
  3. Plant Signal Behav. 2013 Jan;8(1):e22510 - PubMed
  4. PLoS One. 2015 Dec 30;10(12):e0146021 - PubMed
  5. Plant Physiol. 2019 Nov;181(3):855-866 - PubMed
  6. Mol Cell. 2015 Feb 5;57(3):456-66 - PubMed

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