Display options
Cite
Moro B, Kisielow M, Borrero VB, et al. Nuclear RNA purification by flow cytometry to study nuclear processes in plants. STAR Protoc. 2021;2(1):100320doi: 10.1016/j.xpro.2021.100320.
Moro, B., Kisielow, M., Borrero, V. B., Bouet, A., Brosnan, C. A., & Bologna, N. G. (2021). Nuclear RNA purification by flow cytometry to study nuclear processes in plants. STAR protocols, 2(1), 100320. https://doi.org/10.1016/j.xpro.2021.100320
Moro, Belén, et al. "Nuclear RNA purification by flow cytometry to study nuclear processes in plants." STAR protocols vol. 2,1 (2021): 100320. doi: https://doi.org/10.1016/j.xpro.2021.100320
Moro B, Kisielow M, Borrero VB, Bouet A, Brosnan CA, Bologna NG. Nuclear RNA purification by flow cytometry to study nuclear processes in plants. STAR Protoc. 2021 Feb 12;2(1):100320. doi: 10.1016/j.xpro.2021.100320. eCollection 2021 Mar 19. PMID: 33659901; PMCID: PMC7890302.
Copy Download .nbib Format: NLM
Share it on

STAR Protoc. 2021 Feb 12;2(1):100320. doi: 10.1016/j.xpro.2021.100320. eCollection 2021 Mar 19.

Nuclear RNA purification by flow cytometry to study nuclear processes in plants.

STAR protocols

Belén Moro, Malgorzata Kisielow, Veronica Barragan Borrero, Antoine Bouet, Christopher A Brosnan, Nicolás G Bologna

Affiliations

  1. Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Bellaterra, Barcelona 08193, Spain.
  2. ETH Zurich Flow Cytomtery Core Facility, Zurich 8093, Switzerland.
  3. GMI-Gregor Mendel Institute of Molecular Plant Biology (ÖEAW), Vienna 1030, Austria.
  4. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia.

PMID: 33659901 PMCID: PMC7890302 DOI: 10.1016/j.xpro.2021.100320

Abstract

The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on

© 2021 The Authors.

Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Genomics; Model Organisms; RNA-seq

Conflict of interest statement

The authors declare no competing interests.

References

  1. Methods Mol Biol. 2020;2093:95-105 - PubMed
  2. Plant Cell. 2017 Jun;29(6):1248-1261 - PubMed
  3. New Phytol. 2017 Dec;216(4):1002-1017 - PubMed
  4. EMBO J. 2019 Jul 1;38(13):e100754 - PubMed
  5. Curr Biol. 2020 May 4;30(9):1740-1747.e6 - PubMed
  6. Methods Mol Biol. 2014;1112:105-15 - PubMed
  7. Nucleic Acids Res. 2020 Jul 2;48(W1):W553-W557 - PubMed
  8. Nat Commun. 2017 May 17;8:15120 - PubMed
  9. Methods Mol Biol. 2014;1062:509-37 - PubMed
  10. Mol Cell. 2018 Feb 15;69(4):709-719.e5 - PubMed
  11. Methods. 2013 Dec 15;64(3):283-91 - PubMed
  12. Methods. 2014 May 1;67(1):84-90 - PubMed
  13. Cell. 2000 May 26;101(5):555-67 - PubMed
  14. Methods Mol Biol. 2017;1629:27-35 - PubMed
  15. Plant Physiol. 2008 May;147(1):30-40 - PubMed
  16. Plant Physiol. 2005 Sep;139(1):5-17 - PubMed
  17. Nat Protoc. 2011 Jan;6(1):56-68 - PubMed

Publication Types