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Camacho-Martínez P, Martin-Gutiérrez G, Peñalva G, et al. Optimización y adecuación de la capacidad diagnóstica para la realización de grandes volúmenes de RT-PCR de SARS-CoV-2. Optimization and adaptation of the diagnostic capacity for the performance of large volumes of SARS-CoV-2 RT-PCR. Enferm Infecc Microbiol Clin (Engl Ed). 2021;doi: 10.1016/j.eimc.2021.01.014.
Camacho-Martínez, P., Martin-Gutiérrez, G., Peñalva, G., Merino-Díaz, L., & Lepe, J. A. (2021). Optimización y adecuación de la capacidad diagnóstica para la realización de grandes volúmenes de RT-PCR de SARS-CoV-2. Optimization and adaptation of the diagnostic capacity for the performance of large volumes of SARS-CoV-2 RT-PCR. Enfermedades infecciosas y microbiologia clinica (English ed.), . https://doi.org/10.1016/j.eimc.2021.01.014
Camacho-Martínez, Pedro, et al. "Optimización y adecuación de la capacidad diagnóstica para la realización de grandes volúmenes de RT-PCR de SARS-CoV-2." Optimization and adaptation of the diagnostic capacity for the performance of large volumes of SARS-CoV-2 RT-PCR. Enfermedades infecciosas y microbiologia clinica (English ed.) vol. (2021). doi: https://doi.org/10.1016/j.eimc.2021.01.014
Camacho-Martínez P, Martin-Gutiérrez G, Peñalva G, Merino-Díaz L, Lepe JA. Optimización y adecuación de la capacidad diagnóstica para la realización de grandes volúmenes de RT-PCR de SARS-CoV-2. Optimization and adaptation of the diagnostic capacity for the performance of large volumes of SARS-CoV-2 RT-PCR. Enferm Infecc Microbiol Clin (Engl Ed). 2021 Feb 11; doi: 10.1016/j.eimc.2021.01.014. Epub 2021 Feb 11. Spanish. PMID: 33712264; PMCID: PMC7877244.
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Enferm Infecc Microbiol Clin (Engl Ed). 2021 Feb 11; doi: 10.1016/j.eimc.2021.01.014. Epub 2021 Feb 11.

Optimization and adaptation of the diagnostic capacity for the performance of large volumes of SARS-CoV-2 RT-PCR.

Enfermedades infecciosas y microbiologia clinica (English ed.)

[Article in Spanish]
Pedro Camacho-Martínez, Guillermo Martin-Gutiérrez, Germán Peñalva, Laura Merino-Díaz, José Antonio Lepe

Affiliations

  1. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen del Rocío, Sevilla, España.
  2. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen del Rocío, Sevilla, España. Electronic address: [email protected].

PMID: 33712264 PMCID: PMC7877244 DOI: 10.1016/j.eimc.2021.01.014

Abstract

INTRODUCTION: In the present manuscript we describe the planning carried out in our hospital to adapt our diagnostic capability to perform large numbers of SARS-CoV-2 RT-PCR.

METHODS: The analysis and prediction of workflow included the number of RT-PCR per week from the beginning of the pandemic, with a total of 31971 determinations. The planning phase was developed based on the different scenarios previously predicted.

RESULTS: According to the predictions obtained, an automated custom solution was chosen, based on the use of the OT-2 open-source liquid-handling robots (Opentrons), to design a reproducible workflow that achieved a production capacity of 5640 samples/day, with a time of response of four hours per procedure.

CONCLUSIONS: The analysis and prediction of workflow, along with the use of the robotic platforms OT-2, provided a robust structure to deal with the high demand of determinations that this pandemic requires.

Copyright © 2021 Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Publicado por Elsevier España, S.L.U. All rights reserved.

Keywords: Capacidad diagnóstica; Diagnostic capacity; Opentrons; PCR-RT; RT-PCR; SARS-CoV-2

References

  1. Clin Microbiol Infect. 2020 Aug;26(8):1109-1111 - PubMed
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  3. Nat Biotechnol. 2020 Jul;38(7):791-797 - PubMed
  4. N Engl J Med. 2020 Sep 10;383(11):1071-1077 - PubMed

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