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Balog JÁ, Honti V, Kurucz É, et al. Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry. Genomics Proteomics Bioinformatics. 2021;19(2):243-252doi: 10.1016/j.gpb.2020.06.022.
Balog, J. �. �., Honti, V., Kurucz, �. �., Kari, B., Puskás, L. G., Andó, I., & Szebeni, G. J. (2021). Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry. Genomics, proteomics & bioinformatics, 19(2), 243-252. https://doi.org/10.1016/j.gpb.2020.06.022
Balog, József Á, et al. "Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry." Genomics, proteomics & bioinformatics vol. 19,2 (2021): 243-252. doi: https://doi.org/10.1016/j.gpb.2020.06.022
Balog JÁ, Honti V, Kurucz É, Kari B, Puskás LG, Andó I, Szebeni GJ. Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry. Genomics Proteomics Bioinformatics. 2021 Apr;19(2):243-252. doi: 10.1016/j.gpb.2020.06.022. Epub 2021 Mar 10. PMID: 33713850; PMCID: PMC8602394.
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Genomics Proteomics Bioinformatics. 2021 Apr;19(2):243-252. doi: 10.1016/j.gpb.2020.06.022. Epub 2021 Mar 10.

Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry.

Genomics, proteomics & bioinformatics

József Á Balog, Viktor Honti, Éva Kurucz, Beáta Kari, László G Puskás, István Andó, Gábor J Szebeni

Affiliations

  1. Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary; University of Szeged, Ph.D. School in Biology, Szeged H-6726, Hungary.
  2. Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.
  3. Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.
  4. Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary. Electronic address: [email protected].
  5. Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary; Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged H-6726, Hungary. Electronic address: [email protected].

PMID: 33713850 PMCID: PMC8602394 DOI: 10.1016/j.gpb.2020.06.022

Abstract

Single-cell mass cytometry (SCMC) combines features of traditional flow cytometry (i.e., fluorescence-activated cell sorting) with mass spectrometry, making it possible to measure several parameters at the single-cell level for a complex analysis of biological regulatory mechanisms. In this study, weoptimizedSCMC to analyze hemocytes of the Drosophila innate immune system. We used metal-conjugated antibodies (against cell surface antigens H2, H3, H18, L1, L4, and P1, and intracellular antigens 3A5 and L2) and anti-IgM (against cell surface antigen L6) to detect the levels of antigens, while anti-GFP was used to detect crystal cells in the immune-induced samples. We investigated the antigen expression profile of single cells and hemocyte populations in naive states, in immune-induced states, in tumorous mutants bearing a driver mutation in the Drosophila homologue of Janus kinase (hop

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Keywords: Drosophila; Hemocyte; Innate immunity; Mass cytometry; Single-cell analysis

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