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ACS Omega. 2021 Feb 22;6(9):6368-6378. doi: 10.1021/acsomega.0c06299. eCollection 2021 Mar 09.

Efficient Entrapment of Carbonic Anhydrase in Alginate Hydrogels Using Liposomes for Continuous-Flow Catalytic Reactions.

ACS omega

Junshi Moriyama, Makoto Yoshimoto

Affiliations

  1. Department of Applied Chemistry, Yamaguchi University, Tokiwadai 2-16-1, Ube 755-8611, Japan.

PMID: 33718727 PMCID: PMC7948239 DOI: 10.1021/acsomega.0c06299

Abstract

A versatile approach to entrap relatively small enzymes in hydrogels allows their diverse biotechnological applications. In the present work, bovine carbonic anhydrase (BCA) was efficiently entrapped in calcium alginate beads with the help of liposomes. A mixture of sodium alginate (3 wt %) and carbonic anhydrase-liposome conjugates (BCALs) was dripped into a Tris-HCl buffer solution (pH = 7.5) containing 0.4 M CaCl

© 2021 The Authors. Published by American Chemical Society.

Conflict of interest statement

The authors declare no competing financial interest.

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