Display options
Cite
Zhang X, Hu Y, Peng W, et al. Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation. Front Chem. 2021;9:649000doi: 10.3389/fchem.2021.649000.
Zhang, X., Hu, Y., Peng, W., Gao, C., Xing, Q., Wang, B., & Li, A. (2021). Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation. Frontiers in chemistry, 9649000. https://doi.org/10.3389/fchem.2021.649000
Zhang, Xiaodong, et al. "Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation." Frontiers in chemistry vol. 9 (2021): 649000. doi: https://doi.org/10.3389/fchem.2021.649000
Zhang X, Hu Y, Peng W, Gao C, Xing Q, Wang B, Li A. Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation. Front Chem. 2021 Feb 18;9:649000. doi: 10.3389/fchem.2021.649000. eCollection 2021. PMID: 33681151; PMCID: PMC7930613.
Copy Download .nbib Format: NLM
Share it on

Front Chem. 2021 Feb 18;9:649000. doi: 10.3389/fchem.2021.649000. eCollection 2021.

Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio-and Stereoselective Steroid Hydroxylation.

Frontiers in chemistry

Xiaodong Zhang, Yun Hu, Wei Peng, Chenghua Gao, Qiong Xing, Binju Wang, Aitao Li

Affiliations

  1. State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.
  2. State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, National Engineering Laboratory for Green Chemical Productions of Alcohols, Ethers and Esters, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

PMID: 33681151 PMCID: PMC7930613 DOI: 10.3389/fchem.2021.649000

Abstract

Cytochrome P450 enzyme CYP109B1 is a versatile biocatalyst exhibiting hydroxylation activities toward various substrates. However, the regio- and stereoselective steroid hydroxylation by CYP109B1 is far less explored. In this study, the oxidizing activity of CYP109B1 is reconstituted by coupling redox pairs from different sources, or by fusing it to the reductase domain of two self-sufficient P450 enzymes P450RhF and P450BM3 to generate the fused enzyme. The recombinant

Copyright © 2021 Zhang, Hu, Peng, Gao, Xing, Wang and Li.

Keywords: CYP109B1; cytochrome P450; redox partner; regioselectivity; stereoselectivity; steroids hydroxylation

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

  1. Nat Chem. 2017 Jul;9(7):629-634 - PubMed
  2. Angew Chem Int Ed Engl. 2020 Jul 20;59(30):12499-12505 - PubMed
  3. Trends Biotechnol. 2019 Aug;37(8):882-897 - PubMed
  4. Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1353-8 - PubMed
  5. Chembiochem. 2008 Jul 21;9(11):1797-804 - PubMed
  6. Biochemistry. 1995 Jul 4;34(26):8371-9 - PubMed
  7. Photosynth Res. 1988 Oct;18(1-2):179-204 - PubMed
  8. Nat Commun. 2018 Jun 27;9(1):2487 - PubMed
  9. Chem Commun (Camb). 2014 Aug 14;50(63):8771-4 - PubMed
  10. Science. 2013 Jan 18;339(6117):307-10 - PubMed
  11. Nat Prod Rep. 2007 Jun;24(3):585-609 - PubMed
  12. J Biosci Bioeng. 2017 Jun;123(6):692-697 - PubMed
  13. Biochim Biophys Acta. 2007 Mar;1770(3):314-29 - PubMed
  14. Chem Biol. 2008 Jun;15(6):563-72 - PubMed
  15. J Comput Chem. 2004 Jul 15;25(9):1157-74 - PubMed
  16. Biosci Biotechnol Biochem. 2006 Jan;70(1):307-11 - PubMed
  17. Mol Biosyst. 2015 Mar;11(3):869-81 - PubMed
  18. Curr Opin Chem Biol. 2002 Apr;6(2):136-44 - PubMed
  19. Methods Mol Biol. 2017;1645:1-13 - PubMed
  20. J Chem Inf Model. 2016 Apr 25;56(4):599-604 - PubMed
  21. Sci Rep. 2017 Aug 29;7(1):9570 - PubMed
  22. Appl Microbiol Biotechnol. 2010 Jun;87(2):595-607 - PubMed
  23. J Biol Chem. 1964 Jul;239:2370-8 - PubMed
  24. J Chem Theory Comput. 2015 Aug 11;11(8):3696-713 - PubMed
  25. Chem Soc Rev. 2011 Apr;40(4):2003-21 - PubMed
  26. Nat Commun. 2020 Oct 7;11(1):5035 - PubMed
  27. Arch Biochem Biophys. 2003 Jan 1;409(1):59-71 - PubMed
  28. Nucleic Acids Res. 2019 Feb 20;47(3):e15 - PubMed
  29. Steroids. 2009 Nov;74(12):906-12 - PubMed
  30. Chem Sci. 2014 Feb 1;5(2):598-601 - PubMed
  31. J Ind Microbiol Biotechnol. 2017 Feb;44(2):161-166 - PubMed
  32. J Biotechnol. 2006 Jun 25;124(1):128-45 - PubMed
  33. Photosynth Res. 2017 Dec;134(3):265-280 - PubMed
  34. Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):141-154 - PubMed
  35. Appl Microbiol Biotechnol. 2012 Jun;94(6):1423-47 - PubMed
  36. Appl Microbiol Biotechnol. 2014;98(14):6185-203 - PubMed
  37. Nature. 1997 Nov 20;390(6657):249-56 - PubMed
  38. Chem Commun (Camb). 2013 Dec 21;49(98):11572-4 - PubMed
  39. Sci Rep. 2015 Jul 16;5:12158 - PubMed
  40. Microb Cell Fact. 2009 Jul 10;8:36 - PubMed

Publication Types