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Miyoshi M, Imakado Y, Otani L, et al. Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats. Food Sci Nutr. 2021;9(3):1452-1459doi: 10.1002/fsn3.2113.
Miyoshi, M., Imakado, Y., Otani, L., Kaji, M., Aanzai, Y., Sugimoto, N., Murakami, T., Fukuoka, M., Hohjoh, H., Jia, H., & Kato, H. (2021). Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats. Food science & nutrition, 9(3), 1452-1459. https://doi.org/10.1002/fsn3.2113
Miyoshi, Moe, et al. "Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats." Food science & nutrition vol. 9,3 (2021): 1452-1459. doi: https://doi.org/10.1002/fsn3.2113
Miyoshi M, Imakado Y, Otani L, Kaji M, Aanzai Y, Sugimoto N, Murakami T, Fukuoka M, Hohjoh H, Jia H, Kato H. Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats. Food Sci Nutr. 2021 Jan 27;9(3):1452-1459. doi: 10.1002/fsn3.2113. eCollection 2021 Mar. PMID: 33747459; PMCID: PMC7958563.
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Food Sci Nutr. 2021 Jan 27;9(3):1452-1459. doi: 10.1002/fsn3.2113. eCollection 2021 Mar.

Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats.

Food science & nutrition

Moe Miyoshi, Yasuhisa Imakado, Lila Otani, Misa Kaji, Yuki Aanzai, Naoya Sugimoto, Tetsuo Murakami, Masashi Fukuoka, Hirohiko Hohjoh, Huijuan Jia, Hisanori Kato

Affiliations

  1. Health Nutrition Graduate School of Agricultural and Life Sciences University of Tokyo Tokyo Japan.
  2. Department of Food Science and Nutrition Faculty of Agriculture Kinki University Nara Japan.
  3. National Institute of Neuroscience NCNP Tokyo Japan.

PMID: 33747459 PMCID: PMC7958563 DOI: 10.1002/fsn3.2113

Abstract

SCOPE: We previously demonstrated that protein restriction

METHODS AND RESULTS: First, we examined the relation between AT2R DNA methylation and its promoter activity in vitro. Luciferase assays revealed a negative correlation between these two variables. Next, we fed SHRSP dams and grand-dams a control 20% casein diet or a 9% casein diet during pregnancy. Adult offspring and grand-offspring were supplied either water or 1% saline solution for 2 weeks. Renal AT2R promoter DNA near the TATA-box was hypomethylated, mRNA expression was suppressed, and protein expression tended to be higher, in adult offspring of mothers fed a low casein diet. Moreover, adult grand-offspring exhibited high blood pressure after salt loading, along with suppressed transcription of AT2R mRNA and elevated translated protein.

CONCLUSIONS: Under a fetal environment of protein restriction, the increase in protein expression due to hypomethylation of the AT2R promoter region occurs as a response to increased salt sensitivity, and controlling this mechanism may be important for the prevention of hypertension.

© 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.

Keywords: DNA methylation; Stroke‐Prone Spontaneously Hypertensive Rat; angiotensin type 2 receptor; maternal protein restriction; salt sensitivity

Conflict of interest statement

The corresponding author belongs to a university‐community relations laboratory that is associated with Ajinomoto Co., Inc.

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