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S Zibitt M, Hartford CCR, Lal A. Interrogating lncRNA functions via CRISPR/Cas systems. RNA Biol. 2021;18(12):2097-2106doi: 10.1080/15476286.2021.1899500.
S Zibitt, M., Hartford, C. C. R., & Lal, A. (2021). Interrogating lncRNA functions via CRISPR/Cas systems. RNA biology, 18(12), 2097-2106. https://doi.org/10.1080/15476286.2021.1899500
S Zibitt, Meira, et al. "Interrogating lncRNA functions via CRISPR/Cas systems." RNA biology vol. 18,12 (2021): 2097-2106. doi: https://doi.org/10.1080/15476286.2021.1899500
S Zibitt M, Hartford CCR, Lal A. Interrogating lncRNA functions via CRISPR/Cas systems. RNA Biol. 2021 Dec;18(12):2097-2106. doi: 10.1080/15476286.2021.1899500. Epub 2021 Mar 26. PMID: 33685382; PMCID: PMC8632070.
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RNA Biol. 2021 Dec;18(12):2097-2106. doi: 10.1080/15476286.2021.1899500. Epub 2021 Mar 26.

Interrogating lncRNA functions via CRISPR/Cas systems.

RNA biology

Meira S Zibitt, Corrine Corrina R Hartford, Ashish Lal

Affiliations

  1. Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.

PMID: 33685382 PMCID: PMC8632070 DOI: 10.1080/15476286.2021.1899500

Abstract

Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease. Nevertheless, the majority of lncRNAs are low in abundance and poorly conserved, posing challenges to functional studies. The CRISPR/Cas system, an innovative technology that has emerged over the last decade, can be utilized to further understand lncRNA function. The system targets specific DNA and/or RNA sequences via a guide RNA (gRNA) and Cas nuclease complex. We and others have utilized this technology in various applications such as lncRNA knockout, knockdown, overexpression, and imaging. In this review, we summarize how the CRISPR/Cas technology provides new tools to investigate the roles and therapeutic implications of lncRNAs.

Keywords: Lncrna; crispr/cas9; genome editing; lincRNA

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