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Jyothsna KM, Sarkar P, Jha KK, et al. A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate. J Biomed Mater Res A. 2021;109(9):1646-1656doi: 10.1002/jbm.a.37160.
Jyothsna, K. M., Sarkar, P., Jha, K. K., A S, L. K., Raghunathan, V., & Bhat, R. (2021). A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate. Journal of biomedical materials research. Part A, 109(9), 1646-1656. https://doi.org/10.1002/jbm.a.37160
Jyothsna, Konkada Manattayil, et al. "A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate." Journal of biomedical materials research. Part A vol. 109,9 (2021): 1646-1656. doi: https://doi.org/10.1002/jbm.a.37160
Jyothsna KM, Sarkar P, Jha KK, A S LK, Raghunathan V, Bhat R. A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate. J Biomed Mater Res A. 2021 Sep;109(9):1646-1656. doi: 10.1002/jbm.a.37160. Epub 2021 Mar 09. PMID: 33687134.
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J Biomed Mater Res A. 2021 Sep;109(9):1646-1656. doi: 10.1002/jbm.a.37160. Epub 2021 Mar 09.

A biphasic response of polymerized Type 1 collagen architectures to dermatan sulfate.

Journal of biomedical materials research. Part A

Konkada Manattayil Jyothsna, Purba Sarkar, Keshav Kumar Jha, Lal Krishna A S, Varun Raghunathan, Ramray Bhat

Affiliations

  1. Department of Electrical Communication Engineering, Indian Institute of Science, Bangalore, Karnataka, India.
  2. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, India.
  3. Department of Functional Interfaces, Leibniz Institute of Photonic Technology, Jena, Germany.

PMID: 33687134 DOI: 10.1002/jbm.a.37160

Abstract

Collagen I, the most abundant extracellular matrix (ECM) protein in vertebrate tissues provides mechanical durability to tissue microenvironments and regulates cell function. Its fibrillogenesis in biological milieu is predominantly regulated by dermatan sulfate proteoglycans, proteins conjugated with iduronic acid-containing dermatan sulfate (DS) glycosaminoglycans (GAG). Although DS is known to regulate tissue function through its modulation of Coll I architecture, a precise understanding of the latter remains elusive. We investigated this problem by visualizing the fibrillar pattern of fixed Coll I gels polymerized in the presence of varying concentrations of DS using second harmonic generation microscopy. Measuring mean second harmonic generation signal (which estimates the ordering of the fibrils), and surface occupancy (which estimates the space occupied by fibrils) supported by confocal reflectance microscopy, our observations indicated that the effect on fibril pattern of DS is contextual upon the latter's concentrations: Lower levels of DS resulted in sparse disorganized fibrils; higher levels restore organization, with fibrils occupying greater space. An appropriate change in elasticity as a result of DS levels was also observed through atomic force microscopy. Examination of dye-based GAG staining and scanning electron microscopy suggested distinct constitutions of Coll I gels when polymerized with higher and lower levels of DS. We observed that adhesion of the invasive ovarian cancer cells SKOV3 decreased for lower DS levels but was partially restored at higher DS levels. Our study shows how the Coll I gel pattern-tuning of DS is of relevance for understanding its biomaterial applications and possibly, pathophysiological functions.

© 2021 Wiley Periodicals LLC.

Keywords: collagen I; dermatan sulfate; second harmonic generation

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