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Boeckmans J, Cartuyvels R, Hilkens P, et al. Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19. Diagn Microbiol Infect Dis. 2021;100(2):115350doi: 10.1016/j.diagmicrobio.2021.115350.
Boeckmans, J., Cartuyvels, R., Hilkens, P., Bruckers, L., Magerman, K., Waumans, L., & Raymaekers, M. (2021). Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19. Diagnostic microbiology and infectious disease, 100(2), 115350. https://doi.org/10.1016/j.diagmicrobio.2021.115350
Boeckmans, Joost, et al. "Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19." Diagnostic microbiology and infectious disease vol. 100,2 (2021): 115350. doi: https://doi.org/10.1016/j.diagmicrobio.2021.115350
Boeckmans J, Cartuyvels R, Hilkens P, Bruckers L, Magerman K, Waumans L, Raymaekers M. Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19. Diagn Microbiol Infect Dis. 2021 Jun;100(2):115350. doi: 10.1016/j.diagmicrobio.2021.115350. Epub 2021 Feb 18. PMID: 33689985; PMCID: PMC7891066.
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Diagn Microbiol Infect Dis. 2021 Jun;100(2):115350. doi: 10.1016/j.diagmicrobio.2021.115350. Epub 2021 Feb 18.

Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19.

Diagnostic microbiology and infectious disease

Joost Boeckmans, Reinoud Cartuyvels, Petra Hilkens, Liesbeth Bruckers, Koen Magerman, Luc Waumans, Marijke Raymaekers

Affiliations

  1. Clinical Laboratory, Jessa Hospital, Hasselt, Belgium. Electronic address: [email protected].
  2. Clinical Laboratory, Jessa Hospital, Hasselt, Belgium.
  3. I-BioStat, Data Science Institute, Hasselt University, Hasselt, Belgium.
  4. Clinical Laboratory, Jessa Hospital, Hasselt, Belgium; Immunity and Infectious Diseases, Hasselt University, Hasselt, Belgium.

PMID: 33689985 PMCID: PMC7891066 DOI: 10.1016/j.diagmicrobio.2021.115350

Abstract

Detection of SARS-CoV-2 RNA in nasopharyngeal samples using the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the gold standard for diagnosing COVID-19. Determination of SARS-CoV-2 RNA by rRT-PCR sometimes results in an inconclusive test result due to a high cycle threshold-value. We retrospectively analyzed 30,851 SARS-CoV-2 rRT-PCR test results. Borderline positivity was considered as the presence of ≤25 viral copies per milliliter, while no amplification was considered as a negative test result. Of all test results, 204 were answered as borderline, of which 107 were accompanied by a follow-up test within 96 hours. Of the 107 follow-up samples, 10 (9.35%) were found positive for SARS-CoV-2. COVID-19 symptoms were not predictive for testing positive in the follow-up test. The positive SARS-CoV-2 samples in the follow-up group represented 0.92% of all positive test results, highlighting the need for retesting and increased hygienic measures for borderline SARS-CoV-2 patients [NCT04636294].

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Borderline; COVID-19; Molecular diagnostics; SARS-CoV-2; rRT-PCR

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