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de Kok MJC, Schaapherder AF, Wüst RCI, et al. Circumventing the Crabtree effect in cell culture: A systematic review. Mitochondrion. 2021;59:83-95doi: 10.1016/j.mito.2021.03.014.
de Kok, M. J. C., Schaapherder, A. F., Wüst, R. C. I., Zuiderwijk, M., Bakker, J. A., Lindeman, J. H. N., & Le Dévédec, S. E. (2021). Circumventing the Crabtree effect in cell culture: A systematic review. Mitochondrion, 5983-95. https://doi.org/10.1016/j.mito.2021.03.014
de Kok, Michèle J C, et al. "Circumventing the Crabtree effect in cell culture: A systematic review." Mitochondrion vol. 59 (2021): 83-95. doi: https://doi.org/10.1016/j.mito.2021.03.014
de Kok MJC, Schaapherder AF, Wüst RCI, Zuiderwijk M, Bakker JA, Lindeman JHN, Le Dévédec SE. Circumventing the Crabtree effect in cell culture: A systematic review. Mitochondrion. 2021 Jul;59:83-95. doi: 10.1016/j.mito.2021.03.014. Epub 2021 Apr 01. PMID: 33812964.
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Mitochondrion. 2021 Jul;59:83-95. doi: 10.1016/j.mito.2021.03.014. Epub 2021 Apr 01.

Circumventing the Crabtree effect in cell culture: A systematic review.

Mitochondrion

Michèle J C de Kok, Alexander F Schaapherder, Rob C I Wüst, Melissa Zuiderwijk, Jaap A Bakker, Jan H N Lindeman, Sylvia E Le Dévédec

Affiliations

  1. Department of Surgery and Leiden Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  2. Laboratory for Myology, Faculty of Behavioural and Movement Sciences, Amsterdam Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
  3. Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
  4. Department of Clinical Chemistry & Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands.
  5. Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands. Electronic address: [email protected].

PMID: 33812964 DOI: 10.1016/j.mito.2021.03.014

Abstract

Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Keywords: Cell culture; Crabtree effect; Glycolysis; Metabolism; Mitochondria; Oxidative phosphorylation

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