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Amirouche A, Ait-Ali D, Nouri H, et al. TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019. New Microbes New Infect. 2021;41:100874doi: 10.1016/j.nmni.2021.100874.
Amirouche, A., Ait-Ali, D., Nouri, H., Boudrahme-Hannou, L., Tliba, S., Ghidouche, A., Bitam, I., Wright, D., & Renzaho, A. M. N. (2021). TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019. New microbes and new infections, 41100874. https://doi.org/10.1016/j.nmni.2021.100874
Amirouche, A, et al. "TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019." New microbes and new infections vol. 41 (2021): 100874. doi: https://doi.org/10.1016/j.nmni.2021.100874
Amirouche A, Ait-Ali D, Nouri H, Boudrahme-Hannou L, Tliba S, Ghidouche A, Bitam I, Wright D, Renzaho AMN. TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019. New Microbes New Infect. 2021 May;41:100874. doi: 10.1016/j.nmni.2021.100874. Epub 2021 Mar 31. PMID: 33815807; PMCID: PMC8010344.
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New Microbes New Infect. 2021 May;41:100874. doi: 10.1016/j.nmni.2021.100874. Epub 2021 Mar 31.

TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019.

New microbes and new infections

A Amirouche, D Ait-Ali, H Nouri, L Boudrahme-Hannou, S Tliba, A Ghidouche, I Bitam, Wright, Renzaho

Affiliations

  1. Laboratoire de Biochimie Appliquée, CHU de Bejaia, Bejaia, Algeria.
  2. Faculté des Sciences de la Nature et de la Vie, CHU de Bejaia, Bejaia, Algeria.
  3. Laboratoire de Génie Biologique des Cancers, CHU de Bejaia, Bejaia, Algeria.
  4. Laboratoire de Microbiologie Appliquée, CHU de Bejaia, Bejaia, Algeria.
  5. Faculté de Médecine, Université de Bejaia, CHU de Bejaia, Bejaia, Algeria.
  6. Service des maladies infectieuses, CHU de Bejaia, Bejaia, Algeria.
  7. Service de Neurochirurgie, CHU de Bejaia, Bejaia, Algeria.
  8. Ecole Supérieure en Sciences de l'Aliment et des Industries Agroalimentaires (ESSAIA), El Harrach, Alger, Algeria.
  9. Aix-Marseille Université, IRD, VITROME, IHU Méditerranée Infection, Marseille, France.

PMID: 33815807 PMCID: PMC8010344 DOI: 10.1016/j.nmni.2021.100874

Abstract

Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of RNA extraction methods. RNA extraction was performed using three different procedures (TRIzol, QIAamp, VMT-TRIzol) from nine positive SARS-CoV-2 samples. SARS-CoV-2 was detected by real-time reverse transcriptase PCR (RT-PCR) using a detection kit for SARS-CoV-2 (Sun Yat-sen University). Compared to RT-PCR results, there were no discernible differences in detection rates when comparing the three different extraction procedures. On the basis of these results, the use of TRIzol as a transport medium and RNA extraction method for SARS-CoV-2 detection may be a helpful alternative for laboratories facing shortages of commercial testing kits.

© 2021 Published by Elsevier Ltd.

Keywords: Diagnostic techniques; RT-PCR; SARS-CoV-2; TRIzol; throat swab

References

  1. Exp Neurobiol. 2020 Apr 30;29(2):107-119 - PubMed
  2. Clin Infect Dis. 2020 Sep 12;71(6):1547-1551 - PubMed
  3. Nature. 2020 May;581(7809):465-469 - PubMed
  4. Int J Infect Dis. 2020 Aug;97:225-229 - PubMed
  5. Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5439 - PubMed
  6. Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Oct;28(10):1004-8 - PubMed
  7. Lancet. 2020 Feb 15;395(10223):497-506 - PubMed
  8. Clin Infect Dis. 2019 Nov 27;69(12):2170-2176 - PubMed
  9. Virusdisease. 2020 Jun;31(2):97-105 - PubMed
  10. Lancet. 2020 Feb 15;395(10223):507-513 - PubMed
  11. Lancet Respir Med. 2020 May;8(5):475-481 - PubMed
  12. Euro Surveill. 2020 Jan;25(3): - PubMed
  13. N Engl J Med. 2020 Feb 20;382(8):727-733 - PubMed
  14. J Intern Med. 2020 Aug;288(2):192-206 - PubMed
  15. J Equine Sci. 2017;28(4):135-141 - PubMed
  16. BMC Biotechnol. 2014 Nov 14;14:94 - PubMed
  17. J Clin Med. 2020 Feb 26;9(3): - PubMed
  18. Clin Chem. 2020 Apr 1;66(4):549-555 - PubMed
  19. Nat Protoc. 2006;1(2):581-5 - PubMed
  20. Clin Microbiol Infect. 2020 Jul;26(7):842-847 - PubMed
  21. Clin Chem Lab Med. 2003 Aug;41(8):1033-7 - PubMed

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