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Samperio Ventayol P, Geiser P, Di Martino ML, et al. Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers. Proc Natl Acad Sci U S A. 2021;118(16)doi: 10.1073/pnas.2013963118.
Samperio Ventayol, P., Geiser, P., Di Martino, M. L., Florbrant, A., Fattinger, S. A., Walder, N., Sima, E., Shao, F., Gekara, N. O., Sundbom, M., Hardt, W. D., Webb, D. L., Hellström, P. M., Eriksson, J., & Sellin, M. E. (2021). Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers. Proceedings of the National Academy of Sciences of the United States of America, 118(16), . https://doi.org/10.1073/pnas.2013963118
Samperio Ventayol, Pilar, et al. "Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers." Proceedings of the National Academy of Sciences of the United States of America vol. 118,16 (2021). doi: https://doi.org/10.1073/pnas.2013963118
Samperio Ventayol P, Geiser P, Di Martino ML, Florbrant A, Fattinger SA, Walder N, Sima E, Shao F, Gekara NO, Sundbom M, Hardt WD, Webb DL, Hellström PM, Eriksson J, Sellin ME. Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers. Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). doi: 10.1073/pnas.2013963118. PMID: 33846244; PMCID: PMC8072224.
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Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). doi: 10.1073/pnas.2013963118.

Bacterial detection by NAIP/NLRC4 elicits prompt contractions of intestinal epithelial cell layers.

Proceedings of the National Academy of Sciences of the United States of America

Pilar Samperio Ventayol, Petra Geiser, Maria Letizia Di Martino, Alexandra Florbrant, Stefan A Fattinger, Naemi Walder, Eduardo Sima, Feng Shao, Nelson O Gekara, Magnus Sundbom, Wolf-Dietrich Hardt, Dominic-Luc Webb, Per M Hellström, Jens Eriksson, Mikael E Sellin

Affiliations

  1. Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden.
  2. Institute of Microbiology, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland.
  3. Department of Surgical Sciences, Uppsala University, 75185 Uppsala, Sweden.
  4. National Institute of Biological Sciences, 102206 Beijing, China.
  5. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm 10691, Sweden.
  6. Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, 75185 Uppsala, Sweden.
  7. Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden; [email protected].

PMID: 33846244 PMCID: PMC8072224 DOI: 10.1073/pnas.2013963118

Abstract

The gut epithelium serves to maximize the surface for nutrient and fluid uptake, but at the same time must provide a tight barrier to pathogens and remove damaged intestinal epithelial cells (IECs) without jeopardizing barrier integrity. How the epithelium coordinates these tasks remains a question of significant interest. We used imaging and an optical flow analysis pipeline to study the dynamicity of untransformed murine and human intestinal epithelia, cultured atop flexible hydrogel supports. Infection with the pathogen

Copyright © 2021 the Author(s). Published by PNAS.

Keywords: bacterial infection; contraction; epithelium; inflammasome; organoid

Conflict of interest statement

The authors declare no competing interest.

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