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Molho M, Chuang C, Nagy PD. Co-opting of nonATP-generating glycolytic enzymes for TBSV replication. Virology. 2021;559:15-29doi: 10.1016/j.virol.2021.03.011.
Molho, M., Chuang, C., & Nagy, P. D. (2021). Co-opting of nonATP-generating glycolytic enzymes for TBSV replication. Virology, 55915-29. https://doi.org/10.1016/j.virol.2021.03.011
Molho, Melissa, et al. "Co-opting of nonATP-generating glycolytic enzymes for TBSV replication." Virology vol. 559 (2021): 15-29. doi: https://doi.org/10.1016/j.virol.2021.03.011
Molho M, Chuang C, Nagy PD. Co-opting of nonATP-generating glycolytic enzymes for TBSV replication. Virology. 2021 Jul;559:15-29. doi: 10.1016/j.virol.2021.03.011. Epub 2021 Mar 22. PMID: 33799077.
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Virology. 2021 Jul;559:15-29. doi: 10.1016/j.virol.2021.03.011. Epub 2021 Mar 22.

Co-opting of nonATP-generating glycolytic enzymes for TBSV replication.

Virology

Melissa Molho, Chingkai Chuang, Peter D Nagy

Affiliations

  1. Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY, USA.
  2. Department of Plant Pathology, University of Kentucky, Plant Science Building, Lexington, KY, USA. Electronic address: [email protected].

PMID: 33799077 DOI: 10.1016/j.virol.2021.03.011

Abstract

Positive-strand RNA viruses build viral replication organelles (VROs) with the help of co-opted host factors. The energy requirement of intensive viral replication processes is less understood. Previous studies on tomato bushy stunt virus (TBSV) showed that tombusviruses hijack two ATP-producing glycolytic enzymes to produce ATP locally within VROs. In this work, we performed a cDNA library screen with Arabidopsis thaliana proteins and the TBSV p33 replication protein. The p33 - plant interactome contained highly conserved glycolytic proteins. We find that the glycolytic Hxk2 hexokinase, Eno2 phosphopyruvate hydratase and Fba1 fructose 1,6-bisphosphate aldolase are critical for TBSV replication in yeast or in a cell-free replicase reconstitution assay. The recruitment of Fba1 is important for the local production of ATP within VROs. Altogether, our data support the model that TBSV recruits and compartmentalizes possibly most members of the glycolytic pathway. This might allow TBSV to avoid competition with the host for ATP.

Copyright © 2021. Published by Elsevier Inc.

Keywords: ATP generation; ENO2; FBA1; Glycolysis; Hexokinase; Host factor; Replication; Tomato bushy stunt virus; Virus-host interaction; Yeast

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