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Savela ES, Winnett A, Romano AE, et al. SARS-CoV-2 is detectable using sensitive RNA saliva testing days before viral load reaches detection range of low-sensitivity nasal swab tests. medRxiv. 2021;doi: 10.1101/2021.04.02.21254771.
Savela, E. S., Winnett, A., Romano, A. E., Porter, M. K., Shelby, N., Akana, R., Ji, J., Cooper, M. M., Schlenker, N. W., Reyes, J. A., Carter, A. M., Barlow, J. T., Tognazzini, C., Feaster, M., Goh, Y. Y., & Ismagilov, R. F. (2021). SARS-CoV-2 is detectable using sensitive RNA saliva testing days before viral load reaches detection range of low-sensitivity nasal swab tests. medRxiv : the preprint server for health sciences, . https://doi.org/10.1101/2021.04.02.21254771
Savela, Emily S, et al. "SARS-CoV-2 is detectable using sensitive RNA saliva testing days before viral load reaches detection range of low-sensitivity nasal swab tests." medRxiv : the preprint server for health sciences vol. (2021). doi: https://doi.org/10.1101/2021.04.02.21254771
Savela ES, Winnett A, Romano AE, Porter MK, Shelby N, Akana R, Ji J, Cooper MM, Schlenker NW, Reyes JA, Carter AM, Barlow JT, Tognazzini C, Feaster M, Goh YY, Ismagilov RF. SARS-CoV-2 is detectable using sensitive RNA saliva testing days before viral load reaches detection range of low-sensitivity nasal swab tests. medRxiv. 2021 Apr 07; doi: 10.1101/2021.04.02.21254771. PMID: 33851180; PMCID: PMC8043477.
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medRxiv. 2021 Apr 07; doi: 10.1101/2021.04.02.21254771.

SARS-CoV-2 is detectable using sensitive RNA saliva testing days before viral load reaches detection range of low-sensitivity nasal swab tests.

medRxiv : the preprint server for health sciences

Emily S Savela, Alexander Winnett, Anna E Romano, Michael K Porter, Natasha Shelby, Reid Akana, Jenny Ji, Matthew M Cooper, Noah W Schlenker, Jessica A Reyes, Alyssa M Carter, Jacob T Barlow, Colten Tognazzini, Matthew Feaster, Ying-Ying Goh, Rustem F Ismagilov

Affiliations

  1. California Institute of Technology, 1200 E. California Blvd., Pasadena, CA, USA 91125.
  2. City of Pasadena Public Health Department, 1845 N. Fair Oaks Ave., Pasadena, CA, USA 91103.

PMID: 33851180 PMCID: PMC8043477 DOI: 10.1101/2021.04.02.21254771

Abstract

Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and pre-symptomatic spread of COVID-19, curb the spread of viral variants by travelers, and maximize efficacy of therapeutic treatments. We designed a study to evaluate the preferred test sensitivity and sample type (saliva and nasal swab) for detecting early infections of COVID-19. We performed a case-ascertained study to monitor household contacts of individuals recently diagnosed with a SARS-CoV-2 infection. From those individuals, we obtained twice-daily self-collected anterior-nares nasal swabs and saliva samples and quantified SARS-CoV-2 RNA viral loads in those samples using high-sensitivity RT-qPCR and RT-ddPCR assays. We found that SARS-CoV-2 RNA first appears in saliva and then in nasal-swab samples. A high-sensitivity (limit of detection of ~10

Conflict of interest statement

COMPETING INTERESTS STATEMENT The authors report grants from the Bill & Melinda Gates Foundation (to RFI), the Ronald and Maxine Linde Center for New Initiatives at the California Institute of Technol

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