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Simpson RJ, Bonnett GD. Fructan exohydrolase from grasses. New Phytol. 1993;123(3):453-469doi: 10.1111/j.1469-8137.1993.tb03757.x.
Simpson, R. J., & Bonnett, G. D. (1993). Fructan exohydrolase from grasses. The New phytologist, 123(3), 453-469. https://doi.org/10.1111/j.1469-8137.1993.tb03757.x
Simpson, Richard J, and Bonnett, Graham D. "Fructan exohydrolase from grasses." The New phytologist vol. 123,3 (1993): 453-469. doi: https://doi.org/10.1111/j.1469-8137.1993.tb03757.x
Simpson RJ, Bonnett GD. Fructan exohydrolase from grasses. New Phytol. 1993 Mar;123(3):453-469. doi: 10.1111/j.1469-8137.1993.tb03757.x. PMID: 33874127.
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New Phytol. 1993 Mar;123(3):453-469. doi: 10.1111/j.1469-8137.1993.tb03757.x.

Fructan exohydrolase from grasses.

The New phytologist

Richard J Simpson, Graham D Bonnett

Affiliations

  1. School of Agriculture and Forestry, The University of Melbourne, Parkville, 3052, Australia.

PMID: 33874127 DOI: 10.1111/j.1469-8137.1993.tb03757.x

Abstract

In grasses, fructan reserves are mobilized from vegetative plant parts during seasonal growth, after defoliation during grazing and from stems during seed filling. Well-illuminated leaves show a diurnal pattern of fructan accumulation during the light and mobilization during the dark. In expanding leaves, fructans are accumulated in cells of the elongation zone and when mobilized are considered to contribute assimilate for synthetic processes. Even in leaves which do not contain high fructan concentrations, high rates of fructan turnover occur. The process of fructan mobilization appears to be regulated in relation to ontogenic events, demand for assimilate during growth and in response to environmental stress. Hydrolysis of fructans in bacteria is catalyzed by both endo- and exohydrolases. However, in higher plants only fructan exohydrolases (FEH) (EC 3.2.1.80) have been reported. FEH has been extracted from only a limited number of grass species. The pH optimum of FEH activities varies between pH 45-5-5, the temperature optimum ranges from 25-40 °C and FEH is considered to be entirely localized in vacuoles. Estimates of the K

Keywords: Fructan mobilization; fructan exohydrolase (FEH); grasses; invertase; β-fructofuranosidase

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