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Lara-Ochoa C, González-Lara F, Romero-González LE, et al. The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit. Sci Rep. 2021;11(1):8541doi: 10.1038/s41598-021-87586-0.
Lara-Ochoa, C., González-Lara, F., Romero-González, L. E., Jaramillo-Rodríguez, J. B., Vázquez-Arellano, S. I., Medrano-López, A., Cedillo-Ramírez, L., Martínez-Laguna, Y., Girón, J. A., Pérez-Rueda, E., Puente, J. L., & Ibarra, J. A. (2021). The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit. Scientific reports, 11(1), 8541. https://doi.org/10.1038/s41598-021-87586-0
Lara-Ochoa, Cristina, et al. "The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit." Scientific reports vol. 11,1 (2021): 8541. doi: https://doi.org/10.1038/s41598-021-87586-0
Lara-Ochoa C, González-Lara F, Romero-González LE, Jaramillo-Rodríguez JB, Vázquez-Arellano SI, Medrano-López A, Cedillo-Ramírez L, Martínez-Laguna Y, Girón JA, Pérez-Rueda E, Puente JL, Ibarra JA. The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit. Sci Rep. 2021 Apr 20;11(1):8541. doi: 10.1038/s41598-021-87586-0. PMID: 33879812; PMCID: PMC8058060.
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Sci Rep. 2021 Apr 20;11(1):8541. doi: 10.1038/s41598-021-87586-0.

The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit.

Scientific reports

Cristina Lara-Ochoa, Fabiola González-Lara, Luis E Romero-González, Juan B Jaramillo-Rodríguez, Sergio I Vázquez-Arellano, Abraham Medrano-López, Lilia Cedillo-Ramírez, Ygnacio Martínez-Laguna, Jorge A Girón, Ernesto Pérez-Rueda, José Luis Puente, J Antonio Ibarra

Affiliations

  1. Centro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico. [email protected].
  2. Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico. [email protected].
  3. Centro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
  4. Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
  5. Laboratorio de Genética Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
  6. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
  7. Vicerrectoría de Investigación y Estudios de Posgrado, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
  8. Instituto de Investigaciones en Matemáticas Aplicadas y en Sistemas, Universidad Nacional Autónoma de México, Unidad Académica Yucatán, Mérida, Mexico.
  9. Laboratorio de Genética Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico. [email protected].

PMID: 33879812 PMCID: PMC8058060 DOI: 10.1038/s41598-021-87586-0

Abstract

Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.

References

  1. Nucleic Acids Res. 2010 Jul;38(Web Server issue):W576-81 - PubMed
  2. Mol Microbiol. 1996 Apr;20(1):87-100 - PubMed
  3. Anal Chem. 2002 Oct 15;74(20):5383-92 - PubMed
  4. Science. 1995 Dec 1;270(5241):1495-7 - PubMed
  5. J Bacteriol. 2010 Jul;192(14):3597-607 - PubMed
  6. Mol Microbiol. 2004 Mar;51(5):1311-20 - PubMed
  7. Front Microbiol. 2018 Jul 27;9:1694 - PubMed
  8. Curr Opin Microbiol. 2020 Apr;54:67-76 - PubMed
  9. Mol Microbiol. 2004 Nov;54(4):1117-33 - PubMed
  10. PLoS One. 2020 Oct 29;15(10):e0240617 - PubMed
  11. J Mol Biol. 2002 Sep 13;322(2):237-57 - PubMed
  12. Science. 1993 Nov 26;262(5138):1407-13 - PubMed
  13. Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12810-5 - PubMed
  14. J Bacteriol. 1998 Jun;180(11):3013-6 - PubMed
  15. Nat Rev Microbiol. 2004 Feb;2(2):123-40 - PubMed
  16. Front Cell Infect Microbiol. 2017 Feb 07;7:32 - PubMed
  17. J Bacteriol. 2004 Sep;186(18):6277-85 - PubMed
  18. Proteins. 2003 Feb 15;50(3):437-50 - PubMed
  19. J Mol Biol. 1991 Sep 5;221(1):23-9 - PubMed
  20. Nucleic Acids Res. 2016 Jul 8;44(W1):W406-9 - PubMed
  21. J Bacteriol. 1996 May;178(9):2613-28 - PubMed
  22. Science. 1991 Nov 1;254(5032):710-3 - PubMed
  23. Microbiology (Reading). 2010 Apr;156(Pt 4):1120-1133 - PubMed
  24. Int J Proteomics. 2014;2014:147648 - PubMed
  25. Genetica. 2008 May;133(1):65-76 - PubMed
  26. Infect Immun. 1995 May;63(5):1767-76 - PubMed
  27. Mol Microbiol. 1999 Jul;33(1):153-66 - PubMed
  28. FEBS Lett. 2001 Mar 2;491(3):207-11 - PubMed
  29. J Mol Biol. 2000 Jun 23;299(5):1245-55 - PubMed
  30. Genes Dev. 1996 Jan 1;10(1):16-26 - PubMed
  31. J Infect Dis. 1985 Sep;152(3):550-9 - PubMed
  32. Clin Microbiol Infect. 2015 Aug;21(8):729-34 - PubMed
  33. J Bacteriol. 2003 May;185(9):2835-47 - PubMed
  34. Mol Microbiol. 1996 Jan;19(2):307-17 - PubMed
  35. Nat Rev Microbiol. 2008 Jul;6(7):507-19 - PubMed
  36. Infect Immun. 1999 Oct;67(10):5455-62 - PubMed
  37. Annu Rev Genet. 2016 Nov 23;50:493-513 - PubMed
  38. Biochem Biophys Res Commun. 2001 Sep 21;287(2):519-21 - PubMed
  39. J Mol Biol. 1976 Jul 5;104(3):541-55 - PubMed
  40. Mol Microbiol. 2004 Mar;51(5):1297-309 - PubMed
  41. J Bacteriol. 2020 Jun 9;202(13): - PubMed
  42. J Mol Biol. 2012 Jun 8;419(3-4):139-57 - PubMed
  43. Cell Microbiol. 2011 Oct;13(10):1497-517 - PubMed
  44. Nucleic Acids Res. 2019 Jul 2;47(W1):W322-W330 - PubMed
  45. J Bacteriol. 2000 Dec;182(23):6774-82 - PubMed
  46. J Bacteriol. 2002 Oct;184(20):5529-32 - PubMed
  47. Anal Chem. 2003 Sep 1;75(17):4646-58 - PubMed
  48. Mol Microbiol. 2011 Oct;82(2):398-415 - PubMed
  49. J Mol Biol. 2004 Oct 22;343(3):513-32 - PubMed
  50. PLoS One. 2013;8(2):e56977 - PubMed
  51. Nat Rev Microbiol. 2016 Oct;14(10):638-50 - PubMed
  52. Mol Microbiol. 2004 Oct;54(1):45-59 - PubMed
  53. Microbiol Mol Biol Rev. 1997 Dec;61(4):393-410 - PubMed

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