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Meléndez DM, Marti S, Faucitano L, et al. Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit. Animals (Basel). 2021;11(4)doi: 10.3390/ani11040926.
Meléndez, D. M., Marti, S., Faucitano, L., Haley, D. B., Schwinghamer, T. D., & Schwartzkopf-Genswein, K. S. (2021). Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit. Animals : an open access journal from MDPI, 11(4), . https://doi.org/10.3390/ani11040926
Meléndez, Daniela M, et al. "Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit." Animals : an open access journal from MDPI vol. 11,4 (2021). doi: https://doi.org/10.3390/ani11040926
Meléndez DM, Marti S, Faucitano L, Haley DB, Schwinghamer TD, Schwartzkopf-Genswein KS. Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit. Animals (Basel). 2021 Mar 25;11(4). doi: 10.3390/ani11040926. PMID: 33806010; PMCID: PMC8064460.
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Animals (Basel). 2021 Mar 25;11(4). doi: 10.3390/ani11040926.

Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit.

Animals : an open access journal from MDPI

Daniela M Meléndez, Sonia Marti, Luigi Faucitano, Derek B Haley, Timothy D Schwinghamer, Karen S Schwartzkopf-Genswein

Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB T1J 4B1, Canada.
  2. IRTA Ruminant Production Program, Caldes de Montbui, 08140 Barcelona, Spain.
  3. Agriculture and Agri-Food Canada, Sherbrooke Research and Development Centre, Sherbrooke, QC J1M 0C8, Canada.
  4. Department of Population Medicine, University of Guelph, Guelph, ON N1G 2W1, Canada.

PMID: 33806010 PMCID: PMC8064460 DOI: 10.3390/ani11040926

Abstract

Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from

Keywords: L-lactate; analysis; blood; cattle; transport

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