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Masepohl B, Görlitz K, Monnerjahn U, et al. The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation. New Phytol. 1997;136(3):419-423doi: 10.1046/j.1469-8137.1997.00771.x.
Masepohl, B., Görlitz, K., Monnerjahn, U., Moslerand, B., & Böhme, H. (1997). The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation. The New phytologist, 136(3), 419-423. https://doi.org/10.1046/j.1469-8137.1997.00771.x
Masepohl, Bernd, et al. "The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation." The New phytologist vol. 136,3 (1997): 419-423. doi: https://doi.org/10.1046/j.1469-8137.1997.00771.x
Masepohl B, Görlitz K, Monnerjahn U, Moslerand B, Böhme H. The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation. New Phytol. 1997 Jul;136(3):419-423. doi: 10.1046/j.1469-8137.1997.00771.x. PMID: 33863005.
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New Phytol. 1997 Jul;136(3):419-423. doi: 10.1046/j.1469-8137.1997.00771.x.

The ferredoxin-encoding fdxN gene of the filamentous cyanobacterium Anabaena variabilis ATCC 29413 is not essential for nitrogen fixation.

The New phytologist

Bernd Masepohl, Kirsten Görlitz, Ursula Monnerjahn, Britta Moslerand, Herbert Böhme

Affiliations

  1. Botanisches Institut der Universität Bonn, Kirschallee 1, D-53115 Bonn, Germany.

PMID: 33863005 DOI: 10.1046/j.1469-8137.1997.00771.x

Abstract

In contrast to that of Anabaena sp. PCC7120, the fdxN gene in Anabaena variabilis ATCC 29413 is not interrupted by a 55-kb DNA element, making this strain more suitable for genetic analysis of fdxN independent of the developmentally regulated excision during heterocyst formation. As a basis for mutational analysis, the fdxN gene of A. variabilis was cloned and sequenced. The deduced FdxN protein sequence was highly homologous to the Anabaena 7120 fdxN gene product including eight cysteine residues that are known to be conserved among ferredoxins containing two [4Fe-4S] clusters. The fdxN gene of A. variabilis was disrupted by insertion of an interposon within the fdxN coding region resulting in mutant strain KG29. Diazotrophic growth and in vivo nitrogenase activity of KG29 were similar to those of the wild-type, indicating that FdxN was not essential for N

Keywords: 55-kb fdxN DNA element; Anabaena sp.; bacterial-type ferredoxin; heterocysts; nifB-fdxN-nifS-nifU operon

References

  1. Carrasco CD, Ramaswamy KS, Ramasubramanian TS, Golden JW. 1994. Anabaena xisF gene encodes a develop-mentally regulated site-specific recombinase. Genes & Development 8: 74-83. - PubMed
  2. Deng WP, Nickoloff JA. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Analytical Biochemistry 200: 81-88. - PubMed
  3. Elhai J, Wolk CP. 1988a. Conjugal transfer of DNA to cyanobacteria. Methods in Enzymology 167: 747-754. - PubMed
  4. Elhai J, Wolk CP. 1988b. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138. - PubMed
  5. Golden JW, Carrasco CD, Mulligan ME, Schneider GJ, Haselkorn R. 1988. Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. Journal of Bacteriology 170: 5034-5041. - PubMed
  6. Haselkorn R, Buikema WJ. 1992. Nitrogen fixation in cyanobacteria. In: Stacey G, Burris RH, Evans HJ, eds. Biological Nitrogen Fixation. New York , London : Chapman and Hall, 166-190. - PubMed
  7. Jacobson BL, Chae YK, Bohme H, Markley JL, Holden HM. 1992. Crystallization and preliminary analysis of oxidized, recombinant, heterocyst [2Fe-2S] ferredoxin from Anabaena 7120. Archives of Biochemistry and Biophysics 294: 279-281. - PubMed
  8. Jouanneau Y, Meyer C, Naud I, Klipp W. 1995. Characterization of an fdxN mutant of Rhodobacter capsulatus indicates that ferredoxin I serves as electron donor to nitrogenase. Biochimica et Biophysica Acta 1232: 33-42. - PubMed
  9. Lyons EM, Thiel T. 1995. Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase. Journal of Bacteriology 177: 1570-1575. - PubMed
  10. Masepohl B, Klipp W, Puhler A. 1988. Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus. Molecular and General Genetics 212: 27-37. - PubMed
  11. Mulligan ME, Buikema WJ, Haselkorn R. 1988. Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti. Journal of Bacteriology 170: 4406-4410. - PubMed
  12. Mulligan ME, Haselkorn R. 1989. Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120. The nifB-fdxN-nifS-nift) operon. The Journal of Biological Chemistry 264: 19200-19207. - PubMed
  13. Riedel K-U, Jouanneau Y, Masepohl B, Puhler A, Klipp W. 1995. A Rhizobium meliloti ferredoxin (FdxN) purified from Escherichia coli donates electrons to Rhodobacter capsulatus nitrogenase. European Journal of Biochemistry 231: 742-746. - PubMed
  14. Rippka R, Deruelles J, Waterbury JB, Herman M, Stanier RY. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111: 1-61. - PubMed
  15. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. A laboratory manual. Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press. - PubMed
  16. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Acadamy of Sciences USA 74: 5463-5467. - PubMed
  17. Schrautemeier B, Bohme H. 1985. A distinct ferredoxin for nitrogen fixation isolated from heterocysts of the cyanobacterium Anabaena variabilis. Federation of European Biochemical Societies Letters 184: 304-308. - PubMed
  18. Schrautemeier B, Cassing A, Bohme H. 1994. Characterization of the genome region encoding an FdxH-type ferredoxin and a new 2[4Fe-4S] ferredoxin from the non-heterocystous, nitrogen-fixing cyanobacterium Plectonema boryanum PCC 73110. Journal of Bacteriology 176: 1037-1046. - PubMed
  19. Schrautemeier B, Neveling U, Schmitz S. 1995. Distinct and differently regulated Mo-dependent nitrogen-fixing systems evolved for heterocysts and vegetative cells of Anabaena variabilis ATCC 29413: characterization of the fdxHl/2 gene regions as part of the nifl/2 gene clusters. Molecular Microbiology 18: 357-369. - PubMed
  20. Smoker JA, Barnum SR. 1988. Rapid small-scale DNA isolation from filamentous cyanobacteria. FEMS Microbiology Letters 56: 119-122. - PubMed
  21. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185: 60-89. - PubMed
  22. Thiel T. 1993. Characterization of genes for an alternative nitrogenase in the cyanobacterium Anabaena variabilis. Journal of Bacteriology 175: 6276-6286. - PubMed
  23. Thiel T, Lyons EM, Erker JC, Ernst A. 1995. A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. Proceedings of the National Acadamy of Sciences USA 92: 9358-9362. - PubMed
  24. Thomson AJ. 1991. Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II) Federation of European Biochemical Societies Letters 285: 230-236. - PubMed
  25. Wolk CP, Ernst A, Elhai J. 1994. Heterocyst metabolism and development. In: Bryant DA, ed. The Molecular Biology of Cyanobacteria. Dordrecht , Boston , London : Kluwer Academic Publishers, 769-823. - PubMed
  26. Yakunin AF, Hallenbeck PC, Troshina OY, Gogotov IN. 1993. Purification and properties of a bacterial-type ferredoxin from the nitrogen-fixing cyanobacterium Anabaena variabilis ATCC 29413. Biochimica et Biophysica Acta 116: 124-130. - PubMed

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