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Tsurugizawa T, Tamada K, Debacker C, et al. Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI. Bio Protoc. 2021;11(7):e3972doi: 10.21769/BioProtoc.3972.
Tsurugizawa, T., Tamada, K., Debacker, C., Zalesky, A., & Takumi, T. (2021). Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI. Bio-protocol, 11(7), e3972. https://doi.org/10.21769/BioProtoc.3972
Tsurugizawa, Tomokazu, et al. "Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI." Bio-protocol vol. 11,7 (2021): e3972. doi: https://doi.org/10.21769/BioProtoc.3972
Tsurugizawa T, Tamada K, Debacker C, Zalesky A, Takumi T. Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI. Bio Protoc. 2021 Apr 05;11(7):e3972. doi: 10.21769/BioProtoc.3972. eCollection 2021 Apr 05. PMID: 33889666; PMCID: PMC8054203.
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Bio Protoc. 2021 Apr 05;11(7):e3972. doi: 10.21769/BioProtoc.3972. eCollection 2021 Apr 05.

Cranioplastic Surgery and Acclimation Training for Awake Mouse fMRI.

Bio-protocol

Tomokazu Tsurugizawa, Kota Tamada, Clement Debacker, Andrew Zalesky, Toru Takumi

Affiliations

  1. Human Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba-City, Ibaraki, Japan.
  2. NeuroSpin/CEA-Saclay, Gif-sur-Yvette, France.
  3. Department of Physiology and Cell Biology, Kobe University School of Medicine, Chuo, Kobe, Japan.
  4. RIKEN Brain Science Institute, Wako, Saitama, Japan.
  5. Université de Paris, Sorbonne Paris Cité, Paris, France.
  6. Inserm, IMA-Brain, Institut de Psychiatrie et Neurosciences de Paris, Paris, France.
  7. Melbourne Neuropsychiatry Centre and Department of Biomedical Engineering, The University of Melbourne, Victoria, Australia.

PMID: 33889666 PMCID: PMC8054203 DOI: 10.21769/BioProtoc.3972

Abstract

MRI is a promising tool for translational research to link brain function and structure in animal models of disease to patients with neuropsychiatric disorders. However, given that mouse functional MRI (fMRI) typically relies on anesthetics to suppress head motion and physiological noise, it has been difficult to directly compare brain fMRI in anesthetized mice with that in conscious patients. Here, we developed a new system to acquire fMRI in awake mice, which includes a head positioner and dedicated radio frequency coil. The system was used to investigate functional brain networks in conscious mice, with the goal of enabling future studies to bridge fMRI of disease model animals with human fMRI. Cranioplastic surgery was performed to affix the head mount and the cupped-hand handling method was performed to minimize stress during MRI scanning. Here we describe the new mouse fMRI system, cranioplastic surgery and acclimation protocol.

Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.

Keywords: 15q dup mouse ; Autism; Awake fMRI; Diffusion tensor imaging; Functional MRI; Functional connectivity; Structural connectivity

Conflict of interest statement

Competing interestsThe authors declare no competing interests.

References

  1. Cell. 2009 Jun 26;137(7):1235-46 - PubMed
  2. Br J Anaesth. 1993 Nov;71(5):633-41 - PubMed
  3. Brain Sci. 2020 Apr 29;10(5): - PubMed
  4. Sci Adv. 2020 Feb 05;6(6):eaav4520 - PubMed
  5. J Neurosci. 2019 Oct 16;39(42):8347-8361 - PubMed
  6. Sci Rep. 2016 Dec 15;6:38977 - PubMed
  7. Front Neural Circuits. 2017 Feb 03;11:5 - PubMed
  8. Sci Rep. 2019 Feb 27;9(1):2976 - PubMed
  9. J Neurophysiol. 2011 Mar;105(3):1393-405 - PubMed
  10. Cereb Cortex. 2016 Dec;26(12):4497-4512 - PubMed
  11. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11636-41 - PubMed
  12. Neuroscience. 2010 Jan 13;165(1):244-51 - PubMed
  13. Sci Rep. 2020 Mar 3;10(1):4261 - PubMed
  14. Neuroimage. 2014 Nov 15;102 Pt 2:838-47 - PubMed
  15. Eur J Neurosci. 2013 Dec;38(11):3628-35 - PubMed
  16. J Neurosci Methods. 2016 Dec 1;274:38-48 - PubMed
  17. Eur J Neurosci. 2015 Sep;42(5):2125-34 - PubMed
  18. J Neurosci Methods. 2017 Aug 1;287:53-57 - PubMed

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