Display options
Cite
Ferrara MJ, MacArthur TA, Butenas S, et al. Exploring the utility of a novel point-of-care whole blood thrombin generation assay following trauma: A pilot study. Res Pract Thromb Haemost. 2021;5(3):395-402doi: 10.1002/rth2.12483.
Ferrara, M. J., MacArthur, T. A., Butenas, S., Mann, K. G., Immermann, J. M., Spears, G. M., Bailey, K. R., Kozar, R. A., Heller, S. F., Loomis, E. A., Stephens, D., & Park, M. S. (2021). Exploring the utility of a novel point-of-care whole blood thrombin generation assay following trauma: A pilot study. Research and practice in thrombosis and haemostasis, 5(3), 395-402. https://doi.org/10.1002/rth2.12483
Ferrara, Michael J, et al. "Exploring the utility of a novel point-of-care whole blood thrombin generation assay following trauma: A pilot study." Research and practice in thrombosis and haemostasis vol. 5,3 (2021): 395-402. doi: https://doi.org/10.1002/rth2.12483
Ferrara MJ, MacArthur TA, Butenas S, Mann KG, Immermann JM, Spears GM, Bailey KR, Kozar RA, Heller SF, Loomis EA, Stephens D, Park MS. Exploring the utility of a novel point-of-care whole blood thrombin generation assay following trauma: A pilot study. Res Pract Thromb Haemost. 2021 Mar 08;5(3):395-402. doi: 10.1002/rth2.12483. eCollection 2021 Mar. PMID: 33870025; PMCID: PMC8035795.
Copy Download .nbib Format: NLM
Share it on

Res Pract Thromb Haemost. 2021 Mar 08;5(3):395-402. doi: 10.1002/rth2.12483. eCollection 2021 Mar.

Exploring the utility of a novel point-of-care whole blood thrombin generation assay following trauma: A pilot study.

Research and practice in thrombosis and haemostasis

Michael J Ferrara, Taleen A MacArthur, Saulius Butenas, Kenneth G Mann, Joseph M Immermann, Grant M Spears, Kent R Bailey, Rosemary A Kozar, Stephanie F Heller, Erica A Loomis, Daniel Stephens, Myung S Park

Affiliations

  1. Mayo Clinic Rochester MN USA.
  2. University of Vermont Colchester VT USA.
  3. Shock Trauma Center University of Maryland School of Medicine Baltimore MD USA.

PMID: 33870025 PMCID: PMC8035795 DOI: 10.1002/rth2.12483

Abstract

INTRODUCTION: Plasma thrombin generation kinetics as measured by the calibrated automated thrombogram (CAT) assay is a predictor of symptomatic venous thromboembolism after trauma. We hypothesized that data from a new prototype assay for measurement of thrombin generation kinetics in fresh whole blood (near patient testing of thrombin generation), will correlate with the standard CAT assay in the same patients, making it a potential tool in the future care of trauma patients.

METHODS: Patients were enrolled from June 2018 to February 2020. Within 12 hours of injury, blood samples were collected simultaneously for both assays. Variables compared and correlated between assays were lag time, peak height, time to peak, and endogenous thrombin potential. Data are presented as median with interquartile range (IQR). Spearman and Pearson correlations were estimated and tested between both assays; a

RESULTS: A total of 64 trauma patients had samples analyzed: injury severity score = 17 (IQR), 10-26], hospital length of stay = 7.5 (IQR), 2-18) days, age = 52 (IQR, 35-63) years, 71.9% male, and 42.2% of patients received a transfusion within 24 hours of injury. Thrombin generation parameters between plasma and whole blood were compared and found that all parameters of the two assays correlate in trauma patients.

CONCLUSION: In this pilot study, we have found that a novel point-of-care whole blood thrombin generation assay yields results with modest but statistically significant correlations to those of a standard plasma thrombin generation assay. This finding supports studying this device in a larger, adequately powered study.

© 2021 The Authors. Research and Practice in Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis (ISTH).

Keywords: calibrated; kinetics; plasma; thrombin; trauma; venous thromboembolism

References

  1. Shock. 2021 Mar 1;55(3):321-325 - PubMed
  2. Res Pract Thromb Haemost. 2019 Jul 18;3(4):758-768 - PubMed
  3. Thromb Haemost. 2006 Nov;96(5):553-61 - PubMed
  4. Blood. 2016 Aug 25;128(8):1043-9 - PubMed
  5. J Trauma Acute Care Surg. 2015 Nov;79(5):726-31 - PubMed
  6. J Trauma Acute Care Surg. 2017 Sep;83(3):381-387 - PubMed
  7. J Trauma. 2003 Jun;54(6):1127-30 - PubMed
  8. J Thromb Haemost. 2013 Apr 2;: - PubMed
  9. Blood. 2012 Nov 1;120(18):3837-45 - PubMed
  10. Clin Chim Acta. 2015 Feb 20;441:86-9 - PubMed
  11. Crit Care Clin. 2017 Jan;33(1):101-118 - PubMed
  12. Thromb Haemost. 1993 Dec 20;70(6):959-62 - PubMed
  13. Arterioscler Thromb Vasc Biol. 2002 Sep 1;22(9):1381-9 - PubMed
  14. J Trauma Acute Care Surg. 2014 Nov;77(5):674-678 - PubMed
  15. Thromb Haemost. 2006 May;95(5):772-5 - PubMed
  16. Thromb Res. 2016 Aug;144:40-5 - PubMed
  17. Surgery. 2019 Dec;166(6):1122-1127 - PubMed
  18. Vox Sang. 2013 Jul;105(1):11-7 - PubMed
  19. J Thromb Haemost. 2008 Feb;6(2):219-26 - PubMed
  20. J Trauma Acute Care Surg. 2015 Feb;78(2):224-9; discussion 229-30 - PubMed
  21. Curr Opin Anaesthesiol. 2016 Apr;29(2):212-9 - PubMed
  22. Transfusion. 2013 Aug;53(8):1744-54 - PubMed
  23. Thromb Res. 2013 May;131(5):377-82 - PubMed
  24. Surgery. 2012 Jun;151(6):831-6 - PubMed
  25. Hum Reprod. 2013 Jul;28(7):1846-52 - PubMed

Publication Types