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Khoruzhenko A, Miot F, Massart C, et al. Functional model of rat thyroid follicles cultured in Matrigel. Endocr Connect. 2021;10(5):570-578doi: 10.1530/EC-21-0169.
Khoruzhenko, A., Miot, F., Massart, C., Van Sande, J., Dumont, J. E., Beauwens, R., & Boom, A. (2021). Functional model of rat thyroid follicles cultured in Matrigel. Endocrine connections, 10(5), 570-578. https://doi.org/10.1530/EC-21-0169
Khoruzhenko, Antonina, et al. "Functional model of rat thyroid follicles cultured in Matrigel." Endocrine connections vol. 10,5 (2021): 570-578. doi: https://doi.org/10.1530/EC-21-0169
Khoruzhenko A, Miot F, Massart C, Van Sande J, Dumont JE, Beauwens R, Boom A. Functional model of rat thyroid follicles cultured in Matrigel. Endocr Connect. 2021 May 24;10(5):570-578. doi: 10.1530/EC-21-0169. PMID: 33909590; PMCID: PMC8183616.
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Endocr Connect. 2021 May 24;10(5):570-578. doi: 10.1530/EC-21-0169.

Functional model of rat thyroid follicles cultured in Matrigel.

Endocrine connections

Antonina Khoruzhenko, Françoise Miot, Claude Massart, Jacqueline Van Sande, Jacques Emile Dumont, Renaud Beauwens, Alain Boom

Affiliations

  1. Institute of Interdisciplinary Research (IRIBHM), Université libre de Bruxelles, Brussels, Belgium.
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine, Kiev, Ukraine.
  3. Laboratory of Physiology and Pharmacology, Université libre de Bruxelles, Brussels, Belgium.
  4. Laboratoire d'Histologie, de Neuroanatomie et de Neuropathologie, Université libre de Bruxelles, Brussels, Belgium.

PMID: 33909590 PMCID: PMC8183616 DOI: 10.1530/EC-21-0169

Abstract

BACKGROUND: Long-term maintenance of functional activity of thyroid cells is an essential requirement for basic in vitro studies on the physiology and pathology of the thyroid. An important prerequisite of thyrocytes' functional activity in vivo and in vitro is their follicle organization.

AIM: This study aimed at developing a method of cultivation of functionally active rat thyroid follicles in Matrigel under three-dimensional conditions.

METHODS: Undamaged rat thyroid follicles were isolated by enzymatic digestion with collagenase/dispase, then embedded into Matrigel, and cultivated for 2 weeks. Thyroglobulin, thyroxine and zonula occludens-1 (ZO-1) localization were revealed by immunofluorescence analysis. Iodide organification was tested by protein-bound 125I (PBI) measurement.

RESULTS: Integrity of the follicles was preserved during the whole period of cultivation and was confirmed by 3D reconstruction of ZO-1 localization. Thyroglobulin was detected in the thyrocyte cytoplasm, as well as in the intrafollicular lumen. Thyroxine was observed predominantly at the apical side of thyrocytes. Also, generated cultures were characterized by a high level of iodide organification: PB125I represented 39% of the total radioactivity in the Matrigel drop embedding the follicles; at the same time, methimazole almost totally inhibited this process (0.2% of total radioactivity).

CONCLUSION: The method of rat thyrocyte cultivation in Matrigel, as described here allows to maintain the structural integrity and the functional activity of thyroid follicles in vitro and could be used for wide ranges of basic and applied researches in thyroidology.

Keywords: 125I organification; three-dimensional culture of thyroid follicles; thyroglobulin; thyroxine

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