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Morsy FM, Elbadry M, Elbahloul Y. Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock. PeerJ. 2021;9:e11244doi: 10.7717/peerj.11244.
Morsy, F. M., Elbadry, M., & Elbahloul, Y. (2021). Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock. PeerJ, 9e11244. https://doi.org/10.7717/peerj.11244
Morsy, Fatthy Mohamed, et al. "Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock." PeerJ vol. 9 (2021): e11244. doi: https://doi.org/10.7717/peerj.11244
Morsy FM, Elbadry M, Elbahloul Y. Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock. PeerJ. 2021 Apr 19;9:e11244. doi: 10.7717/peerj.11244. eCollection 2021. PMID: 33976974; PMCID: PMC8061573.
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PeerJ. 2021 Apr 19;9:e11244. doi: 10.7717/peerj.11244. eCollection 2021.

Semidry acid hydrolysis of cellulose sustained by autoclaving for production of reducing sugars for bacterial biohydrogen generation from various cellulose feedstock.

PeerJ

Fatthy Mohamed Morsy, Medhat Elbadry, Yasser Elbahloul

Affiliations

  1. Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Almadinah Almunawarah, Saudi Arabia.
  2. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Assiut, Egypt.
  3. Agricultural Microbiology Department, Faculty of Agriculture, Fayoum University, Fayoum, Fayoum, Egypt.
  4. Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Alexandria, Egypt.

PMID: 33976974 PMCID: PMC8061573 DOI: 10.7717/peerj.11244

Abstract

Cellulosic biowastes are one of the cheapest and most abundant renewable organic materials on earth that can be, subsequent to hydrolysis, utilized as an organic carbon source for several fermentation biotechnologies. This study was devoted to explore a semidry acid hydrolysis of cellulose for decreasing the cost and ionic strength of the hydrolysate. For semidry acid hydrolysis, cellulose was just wetted with HCl (0 to 7 M) and subjected to autoclaving. The optimum molar concentration of HCl and period of autoclaving for semidry acid hydrolysis of cellulose were 6 M and 50 min respectively. Subsequent to the semidry acid hydrolysis with a minimum volume of 6 M HCl sustained by autoclaving, the hydrolysate was diluted with distilled water and neutralized with NaOH (0.5 M). The reducing sugars produced from the semidry acid hydrolysis of cellulose was further used for dark fermentation biohydrogen production by

©2021 Morsy et al.

Keywords: Cellulose; Dark fermentation; Escherichia coli; Hydrogen gas; Polysaccharides; Semidry acid hydrolysis

Conflict of interest statement

The authors declare there are no competing interests.

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