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Byrne RAJ, Torvell M, Daskoulidou N, et al. Novel Monoclonal Antibodies Against Mouse C1q: Characterisation and Development of a Quantitative ELISA for Mouse C1q. Mol Neurobiol. 2021;58(9):4323-4336doi: 10.1007/s12035-021-02419-5.
Byrne, R. A. J., Torvell, M., Daskoulidou, N., Fathalla, D., Kokkali, E., Carpanini, S. M., & Morgan, B. P. (2021). Novel Monoclonal Antibodies Against Mouse C1q: Characterisation and Development of a Quantitative ELISA for Mouse C1q. Molecular neurobiology, 58(9), 4323-4336. https://doi.org/10.1007/s12035-021-02419-5
Byrne, Robert A J, et al. "Novel Monoclonal Antibodies Against Mouse C1q: Characterisation and Development of a Quantitative ELISA for Mouse C1q." Molecular neurobiology vol. 58,9 (2021): 4323-4336. doi: https://doi.org/10.1007/s12035-021-02419-5
Byrne RAJ, Torvell M, Daskoulidou N, Fathalla D, Kokkali E, Carpanini SM, Morgan BP. Novel Monoclonal Antibodies Against Mouse C1q: Characterisation and Development of a Quantitative ELISA for Mouse C1q. Mol Neurobiol. 2021 Sep;58(9):4323-4336. doi: 10.1007/s12035-021-02419-5. Epub 2021 May 18. PMID: 34002346; PMCID: PMC8487419.
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Mol Neurobiol. 2021 Sep;58(9):4323-4336. doi: 10.1007/s12035-021-02419-5. Epub 2021 May 18.

Novel Monoclonal Antibodies Against Mouse C1q: Characterisation and Development of a Quantitative ELISA for Mouse C1q.

Molecular neurobiology

Robert A J Byrne, Megan Torvell, Nikoleta Daskoulidou, Dina Fathalla, Eirini Kokkali, Sarah M Carpanini, B Paul Morgan

Affiliations

  1. UK Dementia Research Institute Cardiff, Hadyn Ellis Building, Cardiff University, Maindy Road, Cardiff, CF244HQ, UK.
  2. Division of Infection and Immunity and Systems Immunity Research Institute, School of Medicine, Cardiff University, Hadyn Ellis Building, Heath Park, Cardiff, CF144XN, UK.
  3. School of Optometry and Visual Sciences, Cardiff University, Maindy Road, Cardiff, CF244HQ, UK.
  4. UK Dementia Research Institute Cardiff, Hadyn Ellis Building, Cardiff University, Maindy Road, Cardiff, CF244HQ, UK. [email protected].
  5. Division of Infection and Immunity and Systems Immunity Research Institute, School of Medicine, Cardiff University, Hadyn Ellis Building, Heath Park, Cardiff, CF144XN, UK. [email protected].

PMID: 34002346 PMCID: PMC8487419 DOI: 10.1007/s12035-021-02419-5

Abstract

Recent studies have identified roles for complement in synaptic pruning, both physiological during development and pathological in Alzheimer's disease (AD). These reports suggest that C1q initiates complement activation on synapses and C3 fragments then tag them for removal by microglia. There is an urgent need to characterise these processes in rodent AD models; this requires the development of reagents and methods for detection and quantification of rodent C1q in fluids and pathological tissues. These will enable better evaluation of the role of C1q in disease and its value as disease biomarker. We describe the generation in C1q-deficient mice of novel monoclonal antibodies against mouse and rat C1q that enabled development of a sensitive, specific, and quantitative ELISA for mouse and rat C1q capable of measuring C1q in biological fluids and tissue extracts. Serum C1q levels were measured in wild-type (WT), C1q knockout (KO), C3 KO, C7 KO, Crry KO, and 3xTg and APP

© 2021. The Author(s).

Keywords: Alzheimer’s; C1q; Complement; ELISA; Mouse

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