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Popowski E, Thomson SJ, Knäbel M, et al. Construction of a high density genetic map for hexaploid kiwifruit (Actinidia chinensis var. deliciosa) using genotyping by sequencing. G3 (Bethesda). 2021;doi: 10.1093/g3journal/jkab142.
Popowski, E., Thomson, S. J., Knäbel, M., Tahir, J., Crowhurst, R. N., Davy, M., Foster, T. M., Schaffer, R. J., Tustin, D. S., Allan, A. C., McCallum, J., & Chagné, D. (2021). Construction of a high density genetic map for hexaploid kiwifruit (Actinidia chinensis var. deliciosa) using genotyping by sequencing. G3 (Bethesda, Md.), . https://doi.org/10.1093/g3journal/jkab142
Popowski, Elizabeth, et al. "Construction of a high density genetic map for hexaploid kiwifruit (Actinidia chinensis var. deliciosa) using genotyping by sequencing." G3 (Bethesda, Md.) vol. (2021). doi: https://doi.org/10.1093/g3journal/jkab142
Popowski E, Thomson SJ, Knäbel M, Tahir J, Crowhurst RN, Davy M, Foster TM, Schaffer RJ, Tustin DS, Allan AC, McCallum J, Chagné D. Construction of a high density genetic map for hexaploid kiwifruit (Actinidia chinensis var. deliciosa) using genotyping by sequencing. G3 (Bethesda). 2021 May 01; doi: 10.1093/g3journal/jkab142. Epub 2021 May 01. PMID: 34009255; PMCID: PMC8495948.
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G3 (Bethesda). 2021 May 01; doi: 10.1093/g3journal/jkab142. Epub 2021 May 01.

Construction of a high density genetic map for hexaploid kiwifruit (Actinidia chinensis var. deliciosa) using genotyping by sequencing.

G3 (Bethesda, Md.)

Elizabeth Popowski, Susan J Thomson, Mareika Knäbel, Jibran Tahir, Ross N Crowhurst, Marcus Davy, Toshi M Foster, Robert J Schaffer, D Stuart Tustin, Andrew C Allan, John McCallum, David Chagné

Affiliations

  1. The New Zealand Institute for Plant and Food Research Ltd (Plant & Food Research), Te Puke, New Zealand.
  2. Plant & Food Research, Lincoln, New Zealand.
  3. Plant & Food Research, Palmerston North, New Zealand.
  4. Plant & Food Research, Auckland, New Zealand.
  5. Plant & Food Research, Motueka, New Zealand.
  6. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
  7. Plant & Food Research, Havelock North, New Zealand.

PMID: 34009255 PMCID: PMC8495948 DOI: 10.1093/g3journal/jkab142

Abstract

Commercially grown kiwifruit (genus Actinidia) are generally of two sub-species which have a base haploid genome of 29 chromosomes. The yellow-fleshed A. chinensis var. chinensis, is either diploid (2n = 2x = 58) or tetraploid (2n = 4x = 116) and the green-fleshed cultivar A. chinensis var. deliciosa 'Hayward', is hexaploid (2n = 6x = 174). Advances in breeding green kiwifruit could be greatly sped up by the use of molecular resources for more efficient and faster selection, for example using marker-assisted selection (MAS). The key genetic marker that has been implemented for MAS in hexaploid kiwifruit is for gender testing. Limited marker-trait association has been reported for other polyploid kiwifruit for fruit and production traits. We have constructed a high density linkage map for hexaploid green kiwifruit using genotyping-by-sequence (GBS). The linkage map obtained consists of 3,686 and 3,940 markers organized in 183 and 176 linkage groups for the female and male parents, respectively. Both parental linkage maps are co-linear with the A. chinensis 'Red5' reference genome of kiwifruit. The linkage map was then used for quantitative trait locus (QTL) mapping, and successfully identified QTLs for king flower number, fruit number and weight, dry matter accumulation and storage firmness. These are the first QTLs to be reported and discovered for complex traits in hexaploid kiwifruit.

© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

Keywords: Ericaceae; Linkage mapping; Polyploid; QTLs; SNP markers

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