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Morales-Lange B, Nombela I, Ortega-Villaizán MDM, et al. Induction of . Biology (Basel). 2021;10(4)doi: 10.3390/biology10040324.
Morales-Lange, B., Nombela, I., Ortega-Villaizán, M. D. M., Imarai, M., Schmitt, P., & Mercado, L. (2021). Induction of . Biology, 10(4), . https://doi.org/10.3390/biology10040324
Morales-Lange, Byron, et al. "Induction of ." Biology vol. 10,4 (2021). doi: https://doi.org/10.3390/biology10040324
Morales-Lange B, Nombela I, Ortega-Villaizán MDM, Imarai M, Schmitt P, Mercado L. Induction of . Biology (Basel). 2021 Apr 13;10(4). doi: 10.3390/biology10040324. PMID: 33924548; PMCID: PMC8069158.
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Biology (Basel). 2021 Apr 13;10(4). doi: 10.3390/biology10040324.

Induction of .

Biology

Byron Morales-Lange, Ivan Nombela, María Del Mar Ortega-Villaizán, Mónica Imarai, Paulina Schmitt, Luis Mercado

Affiliations

  1. Grupo de Marcadores Inmunológicos en Organismos Acuáticos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 2340000 Valparaíso, Chile.
  2. Instituto de Biología Molecular y Celular (IBMC) and Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández (UMH), 03202 Elche, Spain.
  3. Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, 3000 Flanders, Belgium.
  4. Centro de Biotecnología Acuícola, Departamento de Biología, Universidad de Santiago de Chile, Estación Central, 9160000 Santiago, Chile.

PMID: 33924548 PMCID: PMC8069158 DOI: 10.3390/biology10040324

Abstract

In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Using these splenocytes, co-cultures of 24 and 48 h are used to determine the gene expression of master transcriptional factors that coordinate the polarization of T cells (

Keywords: Oncorhynchus mykiss; Piscirickettsia salmonis; interferon-gamma; mononuclear cells; transcriptional factors

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