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Morini M, Peli A, Rinnovati R, et al. Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase. Animals (Basel). 2021;11(5)doi: 10.3390/ani11051376.
Morini, M., Peli, A., Rinnovati, R., Magazzù, G., Romagnoli, N., Spadari, A., & Pietra, M. (2021). Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase. Animals : an open access journal from MDPI, 11(5), . https://doi.org/10.3390/ani11051376
Morini, Maria, et al. "Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase." Animals : an open access journal from MDPI vol. 11,5 (2021). doi: https://doi.org/10.3390/ani11051376
Morini M, Peli A, Rinnovati R, Magazzù G, Romagnoli N, Spadari A, Pietra M. Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase. Animals (Basel). 2021 May 12;11(5). doi: 10.3390/ani11051376. PMID: 34066204; PMCID: PMC8151432.
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Animals (Basel). 2021 May 12;11(5). doi: 10.3390/ani11051376.

Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase.

Animals : an open access journal from MDPI

Maria Morini, Angelo Peli, Riccardo Rinnovati, Giuseppe Magazzù, Noemi Romagnoli, Alessandro Spadari, Marco Pietra

Affiliations

  1. Department of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, Italy.
  2. DVM, Vet Practitioner, 40024 Castel San Pietro Terme, 40064 Bologna, Italy.

PMID: 34066204 PMCID: PMC8151432 DOI: 10.3390/ani11051376

Abstract

Severe equine asthma (EA) syndrome is a chronic obstructive disease characterized by exaggerated contraction, inflammation, and structural alteration of the airways in adult horses, when exposed to airborne molds and particulate material. However, little is known about the relationship between the degree and type of inflammation on one hand, and the severity of the disease and the response to treatment on the other. Furthermore, to date, very few studies evaluate the diagnostic value of histology and immunohistochemical features of endoscopic biopsies on subjects with severe equine asthma. To investigate the expression of two inflammatory markers (NKA and IL-8) before, during, and after the exacerbation of severe EA, a histological and immunohistochemical study was carried out on a series of biopsy samples collected by bronchoscopy from six EA-affected horses subjected to process exacerbation through environmental stimuli and then to pharmacological treatment. The application of a histological biopsy scoring system revealed a significant difference between control cases and the EA-affected horses in all experimental phases (asymptomatic, early exacerbation phase, late exacerbation phase, and remission phase). For immunohistochemistry (IHC), only the intensity of NKA positivity increases significantly between control horses and the EA horses at late exacerbation and remission phases. In EA-affected horses, a difference was detected by comparing histology between asymptomatic and remission phase, meanwhile, NKA and IL-8 showed no differences between the experimental phases. Based on these results we can assert that: (1) The endoscopic biopsies generate reliable and homogeneous samples in the entire bronchial tree; (2) the clinical improvement associated with treatment is characterized by a significant worsening of the histological findings; and (3) the NKA immunopositivity seems to increase significantly rather than decrease, as one would have expected, after pharmacological treatment. Further studies are necessary both to implement the number of samples and to use other markers of inflammation to characterize the potential role of cytokines in the diagnosis and therapeutic approach of severe equine asthma.

Keywords: IL-8; NKA; horse; immunohistochemistry; respiratory tract; severe equine asthma

References

  1. Equine Vet J. 2002 Jul;34(4):389-94 - PubMed
  2. Am J Respir Crit Care Med. 2001 Oct 15;164(8 Pt 1):1410-3 - PubMed
  3. Ther Adv Respir Dis. 2009 Oct;3(5):219-26 - PubMed
  4. Front Vet Sci. 2020 Jul 30;7:450 - PubMed
  5. Vet Immunol Immunopathol. 2002 Mar;85(3-4):147-58 - PubMed
  6. J Immunol Methods. 1999 Jan 1;222(1-2):155-69 - PubMed
  7. Am J Vet Res. 2011 Jun;72(6):838-42 - PubMed
  8. BMC Genomics. 2017 Sep 8;18(1):708 - PubMed
  9. Vet Immunol Immunopathol. 1999 Jan 4;67(1):1-15 - PubMed
  10. Br J Pharmacol. 1991 Jun;103(2):1535-41 - PubMed
  11. Anim Genet. 2012 Aug;43(4):450-3 - PubMed
  12. Equine Vet J. 1991 Nov;23(6):434-7 - PubMed
  13. Auton Autacoid Pharmacol. 2002 Aug;22(4):215-24 - PubMed
  14. Mini Rev Med Chem. 2004 Mar;4(3):331-40 - PubMed
  15. Vet Immunol Immunopathol. 2013 Dec 15;156(3-4):190-9 - PubMed
  16. Equine Vet J. 1993 May;25(3):194-7 - PubMed
  17. Eur Respir J. 1997 Aug;10(8):1892-906 - PubMed
  18. Can J Vet Res. 2009 Jan;73(1):25-33 - PubMed
  19. Vet Pathol. 2018 Jan;55(1):144-158 - PubMed
  20. Respirology. 2011 Oct;16(7):1027-46 - PubMed
  21. Vet Clin North Am Equine Pract. 2008 Aug;24(2):423-35, vii-viii - PubMed
  22. Equine Vet J. 2008 Jun;40(4):321-5 - PubMed
  23. Am J Vet Res. 2007 Dec;68(12):1361-9 - PubMed
  24. Vet Res Commun. 2007 Aug;31 Suppl 1:313-6 - PubMed
  25. Vet Clin North Am Equine Pract. 2020 Apr;36(1):87-103 - PubMed
  26. Am J Physiol Lung Cell Mol Physiol. 2007 Apr;292(4):L936-43 - PubMed
  27. Mediators Inflamm. 2016;2016:5693205 - PubMed
  28. J Vet Intern Med. 2006 Jan-Feb;20(1):167-74 - PubMed
  29. Am J Respir Crit Care Med. 1997 Aug;156(2 Pt 1):367-74 - PubMed
  30. Pulm Pharmacol Ther. 2018 Apr;49:88-94 - PubMed
  31. Equine Vet J. 2001 Nov;33(7):730-3 - PubMed
  32. J Am Vet Med Assoc. 1993 Mar 1;202(5):779-82 - PubMed
  33. Equine Vet J. 1998 Sep;30(5):430-4 - PubMed
  34. Equine Vet J. 2014 May;46(3):276-88 - PubMed
  35. J Vet Intern Med. 2016 Mar-Apr;30(2):503-15 - PubMed
  36. J Am Vet Med Assoc. 2000 Feb 15;216(4):554-8 - PubMed
  37. Pol J Vet Sci. 2011;14(3):343-51 - PubMed
  38. Vet Immunol Immunopathol. 2004 Jan;97(1-2):87-96 - PubMed
  39. Equine Vet J. 2001 Mar;33(2):113-5 - PubMed
  40. J Vet Intern Med. 2018 Nov;32(6):2088-2098 - PubMed
  41. Vet Immunol Immunopathol. 2014 Apr 15;158(3-4):238-43 - PubMed
  42. Life Sci. 2004 Feb 6;74(12):1445-63 - PubMed
  43. Vet Clin North Am Equine Pract. 2003 Apr;19(1):63-86, vi - PubMed
  44. Vet J. 1998 Mar;155(2):189-95 - PubMed
  45. Am J Vet Res. 2009 Oct;70(10):1277-83 - PubMed
  46. Vet Immunol Immunopathol. 2005 Oct 18;108(1-2):237-45 - PubMed
  47. Am J Vet Res. 2009 Mar;70(3):365-72 - PubMed
  48. Vet Immunol Immunopathol. 2020 Feb;220:109976 - PubMed
  49. Am J Vet Res. 2018 Mar;79(3):342-347 - PubMed
  50. Vet Immunol Immunopathol. 2003 Nov 15;96(1-2):83-91 - PubMed
  51. Equine Vet J. 2001 May;33(3):244-9 - PubMed
  52. Equine Vet J. 2017 May;49(3):263-265 - PubMed
  53. J Vet Intern Med. 2016 Sep;30(5):1739-1746 - PubMed
  54. Mol Immunol. 2015 Jul;66(1):97-105 - PubMed
  55. Am J Vet Res. 2000 Nov;61(11):1369-74 - PubMed
  56. Vet Immunol Immunopathol. 2008 Apr 15;122(3-4):256-64 - PubMed
  57. Am J Vet Res. 1998 Aug;59(8):1039-43 - PubMed
  58. Am J Vet Res. 2006 Apr;67(4):669-77 - PubMed
  59. Life Sci. 2015 Jun 15;131:44-50 - PubMed
  60. Am J Vet Res. 2005 Sep;66(9):1584-9 - PubMed

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