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Tschesche C, Bekaert M, Humble JL, et al. Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis). Comp Biochem Physiol C Toxicol Pharmacol. 2021;248:109095doi: 10.1016/j.cbpc.2021.109095.
Tschesche, C., Bekaert, M., Humble, J. L., Bron, J. E., & Sturm, A. (2021). Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 248109095. https://doi.org/10.1016/j.cbpc.2021.109095
Tschesche, Claudia, et al. "Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis)." Comparative biochemistry and physiology. Toxicology & pharmacology : CBP vol. 248 (2021): 109095. doi: https://doi.org/10.1016/j.cbpc.2021.109095
Tschesche C, Bekaert M, Humble JL, Bron JE, Sturm A. Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis). Comp Biochem Physiol C Toxicol Pharmacol. 2021 Oct;248:109095. doi: 10.1016/j.cbpc.2021.109095. Epub 2021 Jun 10. PMID: 34098083; PMCID: PMC8387733.
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Comp Biochem Physiol C Toxicol Pharmacol. 2021 Oct;248:109095. doi: 10.1016/j.cbpc.2021.109095. Epub 2021 Jun 10.

Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis).

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Claudia Tschesche, Michaël Bekaert, Joseph L Humble, James E Bron, Armin Sturm

Affiliations

  1. Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom. Electronic address: [email protected].
  2. Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.

PMID: 34098083 PMCID: PMC8387733 DOI: 10.1016/j.cbpc.2021.109095

Abstract

The pyrethroid deltamethrin and the macrocyclic lactone emamectin benzoate (EMB) are used to treat infestations of farmed salmon by parasitic salmon lice, Lepeophtheirus salmonis. While the efficacy of both compounds against Atlantic populations of the parasite has decreased as a result of the evolution of resistance, the molecular mechanisms of drug resistance in L. salmonis are currently not fully understood. The functionally diverse carboxylesterases (CaE) family includes members involved in pesticide resistance phenotypes of terrestrial arthropods. The present study had the objective to characterize the CaE family in L. salmonis and assess its role in drug resistance. L. salmonis CaE homologues were identified by homology searches in the parasite's transcriptome and genome. The transcript expression of CaEs predicted to be catalytically competent was studied using quantitative reverse-transcription PCR in drug susceptible and multi-resistant L. salmonis. The above strategy led to the identification of 21 CaEs genes/pseudogenes. Phylogenetic analyses assigned 13 CaEs to clades involved in neurodevelopmental signaling and cell adhesion, while three sequences were predicted to encode secreted enzymes. Ten CaEs were identified as being potentially catalytically competent. Transcript expression of acetylcholinesterase (ace1b) was significantly increased in multi-resistant lice compared to drug-susceptible L. salmonis, with transcript abundance further increased in preadult-II females following EMB exposure. In summary, results from the present study demonstrate that L. salmonis possesses fewer CaE gene family members than most arthropods characterized so far. Drug resistance in L. salmonis was associated with overexpression of ace1b.

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords: Carboxylesterase; Deltamethrin; Emamectin benzoate; Resistance; Salmon lice

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