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Santos L, Ugun-Klusek A, Coveney C, et al. Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer. NPJ Regen Med. 2021;6(1):32doi: 10.1038/s41536-021-00141-3.
Santos, L., Ugun-Klusek, A., Coveney, C., & Boocock, D. J. (2021). Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer. NPJ Regenerative medicine, 6(1), 32. https://doi.org/10.1038/s41536-021-00141-3
Santos, Lívia, et al. "Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer." NPJ Regenerative medicine vol. 6,1 (2021): 32. doi: https://doi.org/10.1038/s41536-021-00141-3
Santos L, Ugun-Klusek A, Coveney C, Boocock DJ. Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer. NPJ Regen Med. 2021 Jun 07;6(1):32. doi: 10.1038/s41536-021-00141-3. PMID: 34099736; PMCID: PMC8184808.
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NPJ Regen Med. 2021 Jun 07;6(1):32. doi: 10.1038/s41536-021-00141-3.

Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer.

NPJ Regenerative medicine

Lívia Santos, Aslihan Ugun-Klusek, Clare Coveney, David J Boocock

Affiliations

  1. Department of Sport Science, Sport, Health and Performance Enhancement Research Centre (SHAPE), School of Science and Technology, Nottingham Trent University, Nottingham, UK. [email protected].
  2. Department of Biosciences, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham Trent University, Nottingham, UK.
  3. John van Geest Cancer Research Centre, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham Trent University, Nottingham, UK.

PMID: 34099736 PMCID: PMC8184808 DOI: 10.1038/s41536-021-00141-3

Abstract

Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of bone conditions like osteoporosis or metastatic bone cancer. Therefore, it is gaining increasing importance in an emerging area of regenerative medicine-regenerative rehabilitation (RR). Osteocytes are mechanosensitive and secretory bone cells that orchestrate bone anabolism and hence postulated to be an attractive target of regenerative exercise interventions. However, the human osteocyte signalling pathways and processes evoked upon exercise remain to be fully identified. Making use of a computer-controlled bioreactor that mimics exercise and the latest omics approaches, RNA sequencing (RNA-seq) and tandem liquid chromatography-mass spectrometry (LC-MS), we mapped the transcriptome and secretome of mechanically stretched human osteocytic cells. We discovered that a single bout of cyclic stretch activated network processes and signalling pathways likely to modulate bone regeneration and cancer. Furthermore, a comparison between the transcriptome and secretome of stretched human and mouse osteocytic cells revealed dissimilar results, despite both species sharing evolutionarily conserved signalling pathways. These findings suggest that osteocytes can be targeted by exercise-driven RR protocols aiming to modulate bone regeneration or metastatic bone cancer.

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