Display options
Cite
Davies OG, Powell S, Rickard JJ, et al. Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis. J Tissue Eng. 2021;12:20417314211022092doi: 10.1177/20417314211022092.
Davies, O. G., Powell, S., Rickard, J. J., Clancy, M., & Goldberg Oppenheimer, P. (2021). Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis. Journal of tissue engineering, 1220417314211022092. https://doi.org/10.1177/20417314211022092
Davies, Owen G, et al. "Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis." Journal of tissue engineering vol. 12 (2021): 20417314211022092. doi: https://doi.org/10.1177/20417314211022092
Davies OG, Powell S, Rickard JJ, Clancy M, Goldberg Oppenheimer P. Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis. J Tissue Eng. 2021 May 31;12:20417314211022092. doi: 10.1177/20417314211022092. eCollection 2021. PMID: 34104390; PMCID: PMC8172953.
Copy Download .nbib Format: NLM
Share it on

J Tissue Eng. 2021 May 31;12:20417314211022092. doi: 10.1177/20417314211022092. eCollection 2021.

Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis.

Journal of tissue engineering

Owen G Davies, Stephen Powell, Jonathan Js Rickard, Michael Clancy, Pola Goldberg Oppenheimer

Affiliations

  1. School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.
  2. School of Chemical Engineering, University of Birmingham, Birmingham, UK.
  3. Physical Sciences for Health Doctoral Training Centre, University of Birmingham, Birmingham, UK.
  4. Department of Physics, Cavendish Laboratories, University of Cambridge, Cambridge, UK.

PMID: 34104390 PMCID: PMC8172953 DOI: 10.1177/20417314211022092

Abstract

Extracellular vesicles (EVs) hold value as accessible biomarkers for understanding cellular differentiation and related pathologies. Herein, EV biomarkers in models of skeletal muscle dormancy and differentiation have been comparatively profiled using Raman spectroscopy (RS). Significant variations in the biochemical fingerprint of EVs were detected, with an elevation in peaks associated with lipid and protein signatures during early myogenic differentiation (day 2). Principal component analysis revealed a clear separation between the spectra of EVs derived from myogenic and senescent cell types, with non-overlapping interquartile ranges and population median. Observations aligned with nanoparticle tracking data, highlighting a significant early reduction in EV concentration in senescent myoblast cultures as well as notable variations in EV morphology and diameter. As differentiation progressed physical and biochemical differences in the properties of EVs became less pronounced. This study demonstrates the applicability of RS as a high-resolution analytical method for profiling biochemical changes in EVs during early myogenesis.

© The Author(s) 2021.

Keywords: Raman spectroscopy; Vesicle; analytical; differentiation; skeletal muscle

Conflict of interest statement

Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

References

  1. Cell Metab. 2018 Jan 9;27(1):237-251.e4 - PubMed
  2. Nanoscale. 2019 Jan 23;11(4):1661-1679 - PubMed
  3. Sci Rep. 2015 Jun 16;5:11358 - PubMed
  4. Small. 2012 Aug 20;8(16):2595-601 - PubMed
  5. Biomicrofluidics. 2012 Aug 13;6(3):34113 - PubMed
  6. PLoS One. 2014 Jan 02;9(1):e84153 - PubMed
  7. Curr Opin Pharmacol. 2018 Jun;40:147-155 - PubMed
  8. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77 - PubMed
  9. Exp Cell Res. 2010 Jul 15;316(12):1977-84 - PubMed
  10. Chem Cent J. 2013 Feb 25;7(1):37 - PubMed
  11. J Extracell Vesicles. 2012 Nov 21;1: - PubMed
  12. Adv Healthc Mater. 2019 May;8(9):e1801604 - PubMed
  13. Eur J Cell Biol. 1986 Jan;39(2):360-5 - PubMed
  14. Prog Clin Biol Res. 1978;23:563-8 - PubMed
  15. Skelet Muscle. 2018 Jan 31;8(1):3 - PubMed
  16. Theranostics. 2018 Apr 9;8(10):2722-2738 - PubMed
  17. PLoS One. 2015 May 08;10(5):e0125094 - PubMed
  18. FEBS Lett. 2013 May 2;587(9):1379-84 - PubMed
  19. Biochim Biophys Acta Biomembr. 2017 Mar;1859(3):459-466 - PubMed
  20. J Extracell Vesicles. 2015 Dec 07;4:28533 - PubMed
  21. J Extracell Vesicles. 2017 Jun 20;6(1):1329476 - PubMed
  22. Anal Chem. 2018 Oct 2;90(19):11290-11296 - PubMed
  23. PLoS One. 2020 Jul 2;15(7):e0235214 - PubMed
  24. Cell Cycle. 2014;13(1):78-89 - PubMed
  25. J Biol Chem. 2008 Jul 25;283(30):21230-41 - PubMed
  26. Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228 - PubMed
  27. Sci Rep. 2017 Oct 3;7(1):12639 - PubMed
  28. Cells. 2020 Apr 15;9(4): - PubMed
  29. Sci Rep. 2017 Aug 29;7(1):9820 - PubMed
  30. Sci Rep. 2019 Jul 25;9(1):10812 - PubMed
  31. J Extracell Vesicles. 2019 Jan 27;8(1):1568780 - PubMed
  32. Cells. 2020 Jun 02;9(6): - PubMed
  33. ACS Appl Nano Mater. 2020 Jul 24;3(7):6774-6784 - PubMed
  34. J Tissue Eng Regen Med. 2018 Jan;12(1):e355-e367 - PubMed
  35. J Control Release. 2016 Jan 28;222:107-15 - PubMed
  36. Bull World Health Organ. 2012 Feb 1;90(2):82-3 - PubMed
  37. Can J Appl Physiol. 2001 Feb;26(1):90-101 - PubMed
  38. Development. 2014 Apr;141(8):1649-59 - PubMed
  39. PLoS One. 2016 Dec 15;11(12):e0167811 - PubMed
  40. Exp Mol Med. 2020 Jan;52(1):1-6 - PubMed

Publication Types