Reprod Biol Endocrinol. 2021 Jun 09;19(1):86. doi: 10.1186/s12958-021-00770-9.
HSF1 promotes endometriosis development and glycolysis by up-regulating PFKFB3 expression.
Reproductive biology and endocrinology : RB&E
Yixin Wang, Jing Xiu, Tingting Yang, Chune Ren, Zhenhai Yu
Affiliations
Affiliations
- Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China.
- Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China. [email protected].
- Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China. [email protected].
PMID: 34107992
PMCID: PMC8188696 DOI: 10.1186/s12958-021-00770-9
Abstract
BACKGROUND: Endometriosis is a chronic hormonal inflammatory disease characterized by the presence of endometrial tissue outside the uterus. Endometriosis often causes infertility, which brings physical and mental pain to patients and their families.
METHODS: We examined the functions of heat shock factor 1 (HSF1) in endometriosis development through cell count assay, cell-scratch assay and clone formation experiments. We used quantitative real-time PCR (qRT-PCR) and Western blot (WB) to detect HSF1 expression. Glucose and lactate levels were determined using a glucose (GO) assay kit and a lactate assay kit. Furthermore, we used a HSF1 inhibitor-KRIBB11 to establish a mouse model of endometriosis.
RESULTS: Our data demonstrated that HSF1 promoted endometriosis development. Interestingly, HSF1 enhanced glycolysis via up-regulating PFKFB3 expression in endometriosis cells, which was a key glycolysis enzyme. Consistently, the HSF1 inhibitor KRIBB11 could abrogate endometriosis progression in vivo and in vitro.
CONCLUSIONS: Findings indicate that HSF1 plays an important role in endometriosis development, which might become a new target for the treatment of endometriosis.
ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary data are available.
Keywords: Endometriosis; Glycolysis; HSF1; Inhibitor; PFKFB3
References
- Fertil Steril. 2010 May 1;93(7):2433-6 - PubMed
- Cell. 2013 Aug 1;154(3):651-63 - PubMed
- J Proteome Res. 2010 May 7;9(5):2438-49 - PubMed
- Biochim Biophys Acta Rev Cancer. 2020 Aug;1874(1):188390 - PubMed
- Front Physiol. 2018 Jan 23;9:14 - PubMed
- Oncogene. 2018 Nov;37(45):5997-6009 - PubMed
- Trends Pharmacol Sci. 2019 Dec;40(12):986-1005 - PubMed
- Am J Pathol. 2000 Mar;156(3):857-64 - PubMed
- Clin Transl Med. 2021 Apr;11(4):e400 - PubMed
- Theranostics. 2020 Jul 9;10(19):8606-8618 - PubMed
- FEBS J. 2010 Oct;277(20):4140-9 - PubMed
- Cell. 2015 Nov 5;163(4):960-74 - PubMed
- J Clin Endocrinol Metab. 2014 Sep;99(9):3450-9 - PubMed
- Annu Rev Pathol. 2020 Jan 24;15:71-95 - PubMed
- Mol Cancer Res. 2020 May;18(5):787-796 - PubMed
- Oncogene. 2019 Sep;38(38):6537-6549 - PubMed
- Int J Mol Sci. 2020 Apr 17;21(8): - PubMed
- Signal Transduct Target Ther. 2017 Nov 24;2:17044 - PubMed
- Reprod Biomed Online. 2014 Dec;29(6):761-70 - PubMed
- Cell Cycle. 2019 Jun;18(12):1316-1334 - PubMed
- Front Oncol. 2019 Nov 01;9:1143 - PubMed
- Hum Reprod Update. 2020 Apr 15;26(3):412-422 - PubMed
- Int J Mol Sci. 2019 Nov 19;20(22): - PubMed
- Reprod Biol Endocrinol. 2019 Aug 24;17(1):70 - PubMed
- Adv Exp Med Biol. 2020;1243:41-50 - PubMed
- PLoS One. 2012;7(11):e47374 - PubMed
- J Transl Med. 2020 Aug 12;18(1):311 - PubMed
- Br J Cancer. 2014 Jul 8;111(1):78-84 - PubMed
- N Engl J Med. 2010 Jun 24;362(25):2389-98 - PubMed
- J Biol Chem. 2011 Jan 21;286(3):1737-47 - PubMed
- Nat Rev Endocrinol. 2014 May;10(5):261-75 - PubMed
- Mol Metab. 2019 Feb;20:1-13 - PubMed
- Mol Oncol. 2018 May;12(5):690-704 - PubMed
- Endocrinology. 2004 May;145(5):2291-6 - PubMed
- Eur J Med Chem. 2020 Oct 1;203:112612 - PubMed
- Trends Cell Biol. 2016 Jan;26(1):17-28 - PubMed
- Am J Pathol. 1997 Feb;150(2):461-7 - PubMed
- Cancer Res. 2019 Oct 15;79(20):5233-5244 - PubMed
- Curr Cancer Drug Targets. 2019;19(7):515-524 - PubMed
- J Med Life. 2014 Sep 15;7(3):349-57 - PubMed
- Adv Exp Med Biol. 2020;1243:101-111 - PubMed
- Eur J Pharmacol. 2018 Mar 5;822:69-77 - PubMed
- Eur J Obstet Gynecol Reprod Biol. 2021 Mar;258:103-110 - PubMed
- Reprod Sci. 2020 Nov;27(11):1967-1974 - PubMed
- Reprod Sci. 2021 Mar;28(3):728-735 - PubMed
- Science. 1956 Feb 24;123(3191):309-14 - PubMed
- Biomed Res Int. 2013;2013:329058 - PubMed
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