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Keesler DA, Slobodianuk TL, Kochelek CE, et al. Fibronectin binding to von Willebrand factor occurs via the A1 domain. Res Pract Thromb Haemost. 2021;5(5):e12534doi: 10.1002/rth2.12534.
Keesler, D. A., Slobodianuk, T. L., Kochelek, C. E., Skaer, C. W., Haberichter, S. L., & Flood, V. H. (2021). Fibronectin binding to von Willebrand factor occurs via the A1 domain. Research and practice in thrombosis and haemostasis, 5(5), e12534. https://doi.org/10.1002/rth2.12534
Keesler, Daniel A, et al. "Fibronectin binding to von Willebrand factor occurs via the A1 domain." Research and practice in thrombosis and haemostasis vol. 5,5 (2021): e12534. doi: https://doi.org/10.1002/rth2.12534
Keesler DA, Slobodianuk TL, Kochelek CE, Skaer CW, Haberichter SL, Flood VH. Fibronectin binding to von Willebrand factor occurs via the A1 domain. Res Pract Thromb Haemost. 2021 Jun 05;5(5):e12534. doi: 10.1002/rth2.12534. eCollection 2021 Jun. PMID: 34136746; PMCID: PMC8178691.
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Res Pract Thromb Haemost. 2021 Jun 05;5(5):e12534. doi: 10.1002/rth2.12534. eCollection 2021 Jun.

Fibronectin binding to von Willebrand factor occurs via the A1 domain.

Research and practice in thrombosis and haemostasis

Daniel A Keesler, Tricia L Slobodianuk, Caroline E Kochelek, Chad W Skaer, Sandra L Haberichter, Veronica H Flood

Affiliations

  1. Department of Pediatrics Division of Hematology/Oncology Medical College of Wisconsin Milwaukee WI USA.
  2. Blood Research Institute Versiti Wisconsin Milwaukee WI USA.
  3. Children's Research Institute Children's Hospital of Wisconsin Milwaukee WI USA.

PMID: 34136746 PMCID: PMC8178691 DOI: 10.1002/rth2.12534

Abstract

BACKGROUND: Collagen interactions with von Willebrand factor (VWF) perform an important role in initiation of hemostasis.

OBJECTIVES: We hypothesized that in addition to collagen, other extracellular matrix (ECM) proteins such as fibronectin can bind VWF.

METHODS: Fibronectin-VWF interactions were measured by ELISA using both plasma-derived and recombinant VWF-containing variants in specific domains. Inhibition was measured by antibody competition using antibodies directed against both VWF and fibronectin. Binding affinities were measured by the Octet Biosensor for fibronectin and collagen IV.

RESULTS: Fibronectin was able to bind both plasma-derived and recombinant wild-type VWF. This interaction was inhibited by both anti-VWF antibodies and collagen types III and IV. Several VWF A1 domain variants in the region of the collagen IV binding site also demonstrated absent fibronectin binding, as did variants with defects in high-molecular-weight multimers. Binding affinity testing showed fibronectin has a strong affinity for VWF, in a range similar to that of collagen IV. Fibronectin binds VWF via a restricted region of the A1 domain. This interaction requires high-molecular-weight multimers and is similar to that seen with vascular collagens.

CONCLUSIONS: Therefore, VWF would appear to be the common factor linking platelet adhesion to various ECM proteins and facilitating hemostasis under conditions of ECM exposure.

© 2021 The Authors. Research and Practice in Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis (ISTH).

Keywords: collagen; extracellular matrix; fibronectins; hemostasis; von Willebrand factor

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