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Penner AL, Waytt V, Winter T, et al. Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats. Appl Physiol Nutr Metab. 2021;46(11):1378-1388doi: 10.1139/apnm-2021-0161.
Penner, A. L., Waytt, V., Winter, T., Leng, S., Duhamel, T. A., & Aukema, H. M. (2021). Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 46(11), 1378-1388. https://doi.org/10.1139/apnm-2021-0161
Penner, Avery L, et al. "Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats." Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme vol. 46,11 (2021): 1378-1388. doi: https://doi.org/10.1139/apnm-2021-0161
Penner AL, Waytt V, Winter T, Leng S, Duhamel TA, Aukema HM. Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats. Appl Physiol Nutr Metab. 2021 Nov;46(11):1378-1388. doi: 10.1139/apnm-2021-0161. Epub 2021 Jun 11. PMID: 34115947.
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Appl Physiol Nutr Metab. 2021 Nov;46(11):1378-1388. doi: 10.1139/apnm-2021-0161. Epub 2021 Jun 11.

Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats.

Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme

Avery L Penner, Victoria Waytt, Tanja Winter, Shan Leng, Todd A Duhamel, Harold M Aukema

Affiliations

  1. Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
  2. Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Manitoba, Canada.
  3. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Manitoba, Canada.
  4. Faculty of Kinesiology and Recreation Management, University of Manitoba, Winnipeg, Manitoba, Canada.

PMID: 34115947 DOI: 10.1139/apnm-2021-0161

Abstract

Polyunsaturated fatty acids (PUFA)-derived bioactive lipid mediators called oxylipins have been shown to influence muscle growth, inflammation and repair in select muscles. Since individual oxylipins have varying effects and potencies, broad profiling in differing muscle types is required to further understand their overall effects. In addition, diet and sex are key determinants of oxylipin levels. Therefore, to provide comprehensive data on oxylipin profiles in rat soleus (SO), red gastrocnemius (RG), and white gastrocnemius (WG) muscles, female and male weanling Sprague-Dawley rats were provided control or experimental diets enriched in n-3 (ω-3) or n-6 (ω-6) PUFA for 6 weeks. Free oxylipin analysis by HPLC/MS/MS revealed that SO muscle had 25% more oxylipins and 4-13 times greater oxylipin mass than WG muscle. Dietary n-3 PUFA (α-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid) each increased n-3 oxylipins derived directly from their precursors and several that were not direct precursors, while reducing arachidonic acid derived oxylipins. Dietary linoleic acid had few effects on oxylipins. Oxylipins with a sex effect were higher in females in SO and RG. Oxylipins generally reflected the effects of diet and sex on PUFA, but there were exceptions. These fundamental oxylipin profile data provide groundwork knowledge and context for future research on muscle oxylipin functions.

Keywords: PUFA n-3; PUFA n-6; eicosanoids; eicosanoïdes; gastrocnémien blanc; gastrocnémien rouge; muscle squelettique; n-3 PUFA; n-6 PUFA; oxylipines; oxylipins; red gastrocnemius; sex; sexe; skeletal muscle; soleus; soléaire; white gastrocnemius

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