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Olson DG, Maloney M, Lanahan AA, et al. Identifying promoters for gene expression in . Metab Eng Commun. 2015;2:23-29doi: 10.1016/j.meteno.2015.03.002.
Olson, D. G., Maloney, M., Lanahan, A. A., Hon, S., Hauser, L. J., & Lynd, L. R. (2015). Identifying promoters for gene expression in . Metabolic engineering communications, 223-29. https://doi.org/10.1016/j.meteno.2015.03.002
Olson, Daniel G, et al. "Identifying promoters for gene expression in ." Metabolic engineering communications vol. 2 (2015): 23-29. doi: https://doi.org/10.1016/j.meteno.2015.03.002
Olson DG, Maloney M, Lanahan AA, Hon S, Hauser LJ, Lynd LR. Identifying promoters for gene expression in . Metab Eng Commun. 2015 Mar 30;2:23-29. doi: 10.1016/j.meteno.2015.03.002. eCollection 2015 Dec. PMID: 34150505; PMCID: PMC8193255.
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Metab Eng Commun. 2015 Mar 30;2:23-29. doi: 10.1016/j.meteno.2015.03.002. eCollection 2015 Dec.

Identifying promoters for gene expression in .

Metabolic engineering communications

Daniel G Olson, Marybeth Maloney, Anthony A Lanahan, Shuen Hon, Loren J Hauser, Lee R Lynd

Affiliations

  1. Thayer School of Engineering at Dartmouth College, Hanover, NH 03755, United States.
  2. BioEnergy Science Center, TN, United States.
  3. Oak Ridge National Laboratory, Oak Ridge, TN 37830, United States.
  4. Mascoma Corporation, NH 37830, United States.

PMID: 34150505 PMCID: PMC8193255 DOI: 10.1016/j.meteno.2015.03.002

Abstract

A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we test 17 promoter regions for their ability to drive expression of the reporter genes β-galactosidase (

© 2015 The Authors.

Keywords: Copy number; Promoter; Rolling circle replication; Structural instability; adhB; lacZ

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