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Arzumanyan H, Hakobyan S, Avagyan H, et al. Possibility of long-term survival of African swine fever virus in natural conditions. Vet World. 2021;14(4):854-859doi: 10.14202/vetworld.2021.854-859.
Arzumanyan, H., Hakobyan, S., Avagyan, H., Izmailyan, R., Nersisyan, N., & Karalyan, Z. (2021). Possibility of long-term survival of African swine fever virus in natural conditions. Veterinary world, 14(4), 854-859. https://doi.org/10.14202/vetworld.2021.854-859
Arzumanyan, Hranush, et al. "Possibility of long-term survival of African swine fever virus in natural conditions." Veterinary world vol. 14,4 (2021): 854-859. doi: https://doi.org/10.14202/vetworld.2021.854-859
Arzumanyan H, Hakobyan S, Avagyan H, Izmailyan R, Nersisyan N, Karalyan Z. Possibility of long-term survival of African swine fever virus in natural conditions. Vet World. 2021 Apr;14(4):854-859. doi: 10.14202/vetworld.2021.854-859. Epub 2021 Apr 09. PMID: 34083931; PMCID: PMC8167539.
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Vet World. 2021 Apr;14(4):854-859. doi: 10.14202/vetworld.2021.854-859. Epub 2021 Apr 09.

Possibility of long-term survival of African swine fever virus in natural conditions.

Veterinary world

Hranush Arzumanyan, Sona Hakobyan, Hranush Avagyan, Roza Izmailyan, Narek Nersisyan, Zaven Karalyan

Affiliations

  1. Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia.
  2. Experimental Laboratory, Yerevan State Medical University, Yerevan, Armenia.

PMID: 34083931 PMCID: PMC8167539 DOI: 10.14202/vetworld.2021.854-859

Abstract

BACKGROUND AND AIM: In modern scientific literature presents an understanding that African swine fever (ASF) ASF virus (ASFV) is remarkably stable in the environment, and carcasses of the pigs which were died after ASF, play a key role as ASFV reservoir. The aim of this study was to evaluate the possibility of the ASFV (different isolates) survival in bodies of dead animals, bones, remnants of bone marrow, residual organ matrix in natural conditions.

MATERIALS AND METHODS: Skeletons of ASFV infected pigs which were died and left/abandoned in forests or buried in Armenia at diverse time points and locations had been excavated and examined for the presence of ASFV genome by real-time polymerase chain reaction (PCR) assay and for infection abilities through

RESULTS: Current exploration showed that in several samples (with different times of exposure) of excavated skeletons had been detected the presence of the virus gene (p72) using real-time PCR. However, in none of these porcine samples, infectious ASFV could be isolated. Data obtained by real-time PCR at frequent intervals indicated the presence of the virus gene (p72), especially within the case of the acute form of the disease. This can be explained by the highest levels of the virus during the latter case mentioned above.

CONCLUSION: ASFV seems to be very sensitive to environmental temperature. The best place for ASFV long-term survival in the natural environment is bone marrow from intact big tubular bones (like femur or tibia) of buried carcasses. In artificial "graves," complete bones with not destructed bone marrow can preserve the virus gene (p72) for a very long time (more than 2 years). Infectious particles in underground conditions survive not so long: In complete bones with not affected bone marrow, possible presence of the virus for several months.

Copyright: © Arzumanyan, et al.

Keywords: African swine fever virus; p72 gene; virus infectivity; virus survival

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