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Qi CJ, Pan Y, Shen JX, et al. Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside . Ann Clin Lab Sci. 2021;51(3):393-399
Qi, C. J., Pan, Y., Shen, J. X., Kuai, Q. L., Zhu, J. X., & Wang, W. P. (2021). Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside . Annals of clinical and laboratory science, 51(3), 393-399.
Qi, Chao-Jun, et al. "Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside ." Annals of clinical and laboratory science vol. 51,3 (2021): 393-399.
Qi CJ, Pan Y, Shen JX, Kuai QL, Zhu JX, Wang WP. Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside . Ann Clin Lab Sci. 2021 May;51(3):393-399. PMID: 34162570.
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Ann Clin Lab Sci. 2021 May;51(3):393-399.

Inhibition of microRNA-130b Alleviated Podocyte Injury Induced by Puromycin Aminonucleoside .

Annals of clinical and laboratory science

Chao-Jun Qi, Yan Pan, Jian-Xiao Shen, Qiao-Lin Kuai, Jin-Xin Zhu, Wan-Peng Wang

Affiliations

  1. Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
  2. Department of Clinical Laboratory, Lianshui People's Hospital, Kangda college of Nanjing Medical University, Huai'an city, Jiangsu, China.
  3. Department of Nephrology, Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an city, Jiangsu, China.
  4. Department of Central Laboratory, Lianshui People's Hospital, Kangda college of Nanjing Medical University, Huai'an city, Jiangsu, China.
  5. Department of Nephrology, Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an city, Jiangsu, China [email protected].

PMID: 34162570

Abstract

OBJECTIVE: To investigate the effect of microRNA-130b (miR-130b) on podocyte injury induced by puromycin aminonucleoside (PAN) and its possible mechanisms.

METHODS: The immortalized podocytes (HPC) were treated by 25, 50, or 100 μg/mL PAN, then real-time polymerase chain reaction (PCR) was used to detect the expression of miR-130b. The HPC were transfected with miR-130b inhibitor or normal control (NC) inhibitor, and then the cells were stimulated with 100 μg/mL PAN for 24h. Western blot was used to detect the protein expression of synaptopodin and nephrin. Phalloidin dying was used to detect the changes in the cytoskeleton. Flow cytometry was used to measure podocyte apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR-130b and PGC1α.

RESULTS: PAN significantly upregulated the expression of miR-130b. The western blot showed that inhibition of miR-130b increased the protein expression of synaptopodin and nephrin compared to the negative control inhibitor group. The phalloidin dying showed that inhibition of miR-130b alleviated cytoskeletal remodeling of podocytes induced by PAN. Flow-cytometric analysis showed that apoptosis was decreased after miR-130b silencing. The miR-130b mimic could significantly down-regulate the protein expression of PGC1α, and the dual luciferase reporter assay showed that miR-130b induced a decrease in PGC1α 3'-UTR luciferase activity compared to the control mimic group, but there was no significant difference between the control mimic group and the mut·PGC1α 3'-UTR group.

CONCLUSION: miR-130b ameliorates podocyte injury induced by PAN through inhibiting the expression of PGC1α.

© 2021 by the Association of Clinical Scientists, Inc.

Keywords: Chronic Kidney Diseases (CKD); Peroxisome Proliferator-activated Receptor Gamma Coactivator (PGC1α); Podocyte; Proteinuria; microRNA-130b

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