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Hao N, Chen Q, Dodd IB, et al. The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in . ACS Synth Biol. 2021;10(7):1633-1639doi: 10.1021/acssynbio.1c00215.
Hao, N., Chen, Q., Dodd, I. B., & Shearwin, K. E. (2021). The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in . ACS synthetic biology, 10(7), 1633-1639. https://doi.org/10.1021/acssynbio.1c00215
Hao, Nan, et al. "The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in ." ACS synthetic biology vol. 10,7 (2021): 1633-1639. doi: https://doi.org/10.1021/acssynbio.1c00215
Hao N, Chen Q, Dodd IB, Shearwin KE. The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in . ACS Synth Biol. 2021 Jul 16;10(7):1633-1639. doi: 10.1021/acssynbio.1c00215. Epub 2021 Jun 30. PMID: 34190535.
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ACS Synth Biol. 2021 Jul 16;10(7):1633-1639. doi: 10.1021/acssynbio.1c00215. Epub 2021 Jun 30.

The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in .

ACS synthetic biology

Nan Hao, Qinqin Chen, Ian B Dodd, Keith E Shearwin

Affiliations

  1. Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
  2. CSIRO Synthetic Biology Future Science Platform, Canberra, ACT 2601, Australia.

PMID: 34190535 DOI: 10.1021/acssynbio.1c00215

Abstract

We describe a new set of tools for inserting DNA into the bacterial chromosome. The system uses site-specific recombination reactions carried out by bacteriophage integrases to integrate plasmids at up to eight phage attachment sites in

Keywords: Cre/loxP; chromosomal integration; cosmid transduction; genome engineering

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