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Maher M, Castilho M, Yue Z, et al. Shaping collagen for engineering hard tissues: Towards a printomics approach. Acta Biomater. 2021;131:41-61doi: 10.1016/j.actbio.2021.06.035.
Maher, M., Castilho, M., Yue, Z., Glattauer, V., Hughes, T. C., Ramshaw, J. A. M., & Wallace, G. G. (2021). Shaping collagen for engineering hard tissues: Towards a printomics approach. Acta biomaterialia, 13141-61. https://doi.org/10.1016/j.actbio.2021.06.035
Maher, Malachy, et al. "Shaping collagen for engineering hard tissues: Towards a printomics approach." Acta biomaterialia vol. 131 (2021): 41-61. doi: https://doi.org/10.1016/j.actbio.2021.06.035
Maher M, Castilho M, Yue Z, Glattauer V, Hughes TC, Ramshaw JAM, Wallace GG. Shaping collagen for engineering hard tissues: Towards a printomics approach. Acta Biomater. 2021 Sep 01;131:41-61. doi: 10.1016/j.actbio.2021.06.035. Epub 2021 Jun 27. PMID: 34192571.
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Acta Biomater. 2021 Sep 01;131:41-61. doi: 10.1016/j.actbio.2021.06.035. Epub 2021 Jun 27.

Shaping collagen for engineering hard tissues: Towards a printomics approach.

Acta biomaterialia

Malachy Maher, Miguel Castilho, Zhilian Yue, Veronica Glattauer, Timothy C Hughes, John A M Ramshaw, Gordon G Wallace

Affiliations

  1. Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, Innovation Campus, University of Wollongong, NSW 2519, Australia; Commonwealth Scientific Industrial Research Organisation, Manufacturing Clayton, VIC 3168, Australia.
  2. Department of Orthopaedics, University Medical Centre Utrecht, Utrecht University, Utrecht, the Netherlands; Regenerative Medicine Centre, Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Biomedical Engineering, Faculty of Engineering, Technical University of Eindhoven, The Netherlands. Electronic address: [email protected].
  3. Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, Innovation Campus, University of Wollongong, NSW 2519, Australia.
  4. Commonwealth Scientific Industrial Research Organisation, Manufacturing Clayton, VIC 3168, Australia.
  5. Department of Surgery, St. Vincent's Hospital, University of Melbourne, VIC 3065, Australia.
  6. Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, Innovation Campus, University of Wollongong, NSW 2519, Australia. Electronic address: [email protected].

PMID: 34192571 DOI: 10.1016/j.actbio.2021.06.035

Abstract

Hard tissue engineering has evolved over the past decades, with multiple approaches being explored and developed. Despite the rapid development and success of advanced 3D cell culture, 3D printing technologies and material developments, a gold standard approach to engineering and regenerating hard tissue substitutes such as bone, dentin and cementum, has not yet been realised. One such strategy that differs from conventional regenerative medicine approach of other tissues, is the in vitro mineralisation of collagen templates in the absence of cells. Collagen is the most abundant protein within the human body and forms the basis of all hard tissues. Once mineralised, collagen provides important support and protection to humans, for example in the case of bone tissue. Multiple in vitro fabrication strategies and mineralisation approaches have been developed and their success in facilitating mineral deposition on collagen to achieve bone-like scaffolds evaluated. Critical to the success of such fabrication and biomineralisation approaches is the collagen template, and its chemical composition, organisation, and density. The key factors that influence such properties are the collagen processing and fabrication techniques utilised to create the template, and the mineralisation strategy employed to deposit mineral on and throughout the templates. However, despite its importance, relatively little attention has been placed on these two critical factors. Here, we critically examine the processing, fabrication and mineralisation strategies that have been used to mineralise collagen templates, and offer insights and perspectives on the most promising strategies for creating mineralised collagen scaffolds. STATEMENT OF SIGNIFICANCE: In this review, we highlight the critical need to fabricate collagen templates with advanced processing techniques, in a manner that achieves biomimicry of the hierarchical collagen structure, prior to utilising in vitro mineralisation strategies. To this end, we focus on the initial collagen that is selected, the extraction techniques used and the native fibril forming potential retained to create reconstituted collagen scaffolds. This review synthesises current best practises in material sourcing, processing, mineralisation strategies and fabrication techniques, and offers insights into how these can best be exploited in future studies to successfully mineralise collagen templates.

Copyright © 2021. Published by Elsevier Ltd.

Keywords: 3D printing; Biomimicry; Collagen; Hierarchical; Mineralisation

Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this pa

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