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de Souza-Rodrigues RD, Puty B, Bonfim L, et al. Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ. 2021;9:e11114doi: 10.7717/peerj.11114.
de Souza-Rodrigues, R. D., Puty, B., Bonfim, L., Nogueira, L. S., Nascimento, P. C., Bittencourt, L. O., Couto, R. S. D., Barboza, C. A. G., de Oliveira, E. H. C., Marques, M. M., & Lima, R. R. (2021). Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ, 9e11114. https://doi.org/10.7717/peerj.11114
de Souza-Rodrigues, Renata Duarte, et al. "Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings." PeerJ vol. 9 (2021): e11114. doi: https://doi.org/10.7717/peerj.11114
de Souza-Rodrigues RD, Puty B, Bonfim L, Nogueira LS, Nascimento PC, Bittencourt LO, Couto RSD, Barboza CAG, de Oliveira EHC, Marques MM, Lima RR. Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ. 2021 Jun 10;9:e11114. doi: 10.7717/peerj.11114. eCollection 2021. PMID: 34178433; PMCID: PMC8199917.
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PeerJ. 2021 Jun 10;9:e11114. doi: 10.7717/peerj.11114. eCollection 2021.

Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.

PeerJ

Renata Duarte de Souza-Rodrigues, Bruna Puty, Laís Bonfim, Lygia Sega Nogueira, Priscila Cunha Nascimento, Leonardo Oliveira Bittencourt, Roberta Souza D'Almeida Couto, Carlos Augusto Galvão Barboza, Edivaldo Herculano Corrêa de Oliveira, Marcia Martins Marques, Rafael Rodrigues Lima

Affiliations

  1. Institute of Arts Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, Brazil.
  2. Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, Brazil.
  3. Laboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua, Pará, Brazil.
  4. Faculty of Dentistry, Federal University of Pará (UFPA), Belém, Pará, Brazil.
  5. Department of Morphology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
  6. Graduation Program, School of Dentistry, Ibirapuera University (UNIb), São Paulo, Brazil.

PMID: 34178433 PMCID: PMC8199917 DOI: 10.7717/peerj.11114

Abstract

BACKGROUND: Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure.

METHODS: Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 µM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 µM MeHg were tested by measuring glutathione (GSH) level.

RESULTS: It was observed that cell viability decreased significantly after exposure to 2.5 and 5 µM of MeHg, but the metabolic viability only decreased significantly at 5 µM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 µM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells.

© 2021 Souza-Rodrigues et al.

Keywords: Cell metabolism; Cell viability; Dental pulp stem cells; Methylmercury (MeHg); Oxidative stress

Conflict of interest statement

The authors declare that they have no competing interests.

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