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Rütgen BC, Baumgartner D, Fuchs-Baumgartinger A, et al. Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes. Front Vet Sci. 2021;8:663656doi: 10.3389/fvets.2021.663656.
Rütgen, B. C., Baumgartner, D., Fuchs-Baumgartinger, A., Rigillo, A., Škor, O., Hammer, S. E., Saalmüller, A., & Schwendenwein, I. (2021). Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes. Frontiers in veterinary science, 8663656. https://doi.org/10.3389/fvets.2021.663656
Rütgen, Barbara C, et al. "Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes." Frontiers in veterinary science vol. 8 (2021): 663656. doi: https://doi.org/10.3389/fvets.2021.663656
Rütgen BC, Baumgartner D, Fuchs-Baumgartinger A, Rigillo A, Škor O, Hammer SE, Saalmüller A, Schwendenwein I. Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes. Front Vet Sci. 2021 Jun 29;8:663656. doi: 10.3389/fvets.2021.663656. eCollection 2021. PMID: 34268346; PMCID: PMC8276100.
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Front Vet Sci. 2021 Jun 29;8:663656. doi: 10.3389/fvets.2021.663656. eCollection 2021.

Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes.

Frontiers in veterinary science

Barbara C Rütgen, Daniel Baumgartner, Andrea Fuchs-Baumgartinger, Antonella Rigillo, Ondřej Škor, Sabine E Hammer, Armin Saalmüller, Ilse Schwendenwein

Affiliations

  1. Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
  2. Institute of Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
  3. Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
  4. Clinic for Internal Medicine, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria.
  5. Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.

PMID: 34268346 PMCID: PMC8276100 DOI: 10.3389/fvets.2021.663656

Abstract

Recent literature suggests conventional flow cytometric (FCM) immunophenotyping complemented by Ki-67 FCM assessment as a reliable tool to classify canine lymphomas. Ki-67 expression assessed by FCM is rarely reported in canine lymphoma cases and reference data for normal canine lymph nodes are missing. Moreover, nothing is known about the Ki-67 expression within the occasionally observed remnant cell population within the gates of normal lymphocytes in lymphoma cases. Aim of this study was to compare flow cytometric Ki-67 expression of lymphocyte populations from normal canine lymph nodes, lymphoma cells from World-Health-Organisation (WHO) classified lymphoma patient samples and their neighboring normal remnant cell population. Cryopreserved lymphocyte cell suspensions from normal lymph nodes from eight dogs free of lymphoma served as reference material. Fourteen cases diagnosed by cytology, FCM, clonality testing, histopathology including immunohistochemistry consisting of 10 DLBCL, 1 MZL, 1 PTCL and 2 TZL showed a residual small lymphocyte population and were investigated. The Ki-67 expression in normal canine lymphoid tissue was 3.19 ± 2.17%. Mean Ki-67 expression in the malignant cell populations was 41 ± 24.36%. Ki-67 positivity was 12.34 ± 10.68% in the residual physiologic lymphocyte population, which otherwise exhibited a physiologic immunophenotype pattern. This ratio was equivalent (

Copyright © 2021 Rütgen, Baumgartner, Fuchs-Baumgartinger, Rigillo, Škor, Hammer, Saalmüller and Schwendenwein.

Keywords: Ki-67; canine lymphoma; flow cytometry; immunophenotyping; reference data

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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