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Appl Spectrosc. 2021 Dec;75(12):1461-1464. doi: 10.1177/00037028211029312. Epub 2021 Jul 16.

Enhanced Fluorescence in a Scattering Medium.

Applied spectroscopy

Nathan A Giauque, Callum E Flowerday, Steven R Goates

Affiliations

  1. Department of Chemistry and Biochemistry, Brigham Young University, Provo, USA.

PMID: 34269092 DOI: 10.1177/00037028211029312

Abstract

Often only small amounts of sample are available for spectroscopic analytical determinations. This work investigates the enhancement of signal in columns packed with silica particles. We propose that silica particles cause the light to scatter through the column, effectively increasing optical path length. Packed columns are shown to be effective with fluorescence spectroscopy, but results were inconclusive with absorbance spectroscopy.

Keywords: Small volume; enhanced detection; fluorescence; packed column; scattering

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