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Sinha I, Modesto J, Krebs NM, et al. Changes in salivary proteome before and after cigarette smoking in smokers compared to sham smoking in nonsmokers: A pilot study. Tob Induc Dis. 2021;19:56doi: 10.18332/tid/138336.
Sinha, I., Modesto, J., Krebs, N. M., Stanley, A. E., Walter, V. A., Richie, J. P., Muscat, J. E., & Sinha, R. (2021). Changes in salivary proteome before and after cigarette smoking in smokers compared to sham smoking in nonsmokers: A pilot study. Tobacco induced diseases, 1956. https://doi.org/10.18332/tid/138336
Sinha, Indu, et al. "Changes in salivary proteome before and after cigarette smoking in smokers compared to sham smoking in nonsmokers: A pilot study." Tobacco induced diseases vol. 19 (2021): 56. doi: https://doi.org/10.18332/tid/138336
Sinha I, Modesto J, Krebs NM, Stanley AE, Walter VA, Richie JP, Muscat JE, Sinha R. Changes in salivary proteome before and after cigarette smoking in smokers compared to sham smoking in nonsmokers: A pilot study. Tob Induc Dis. 2021 Jun 29;19:56. doi: 10.18332/tid/138336. eCollection 2021. PMID: 34239408; PMCID: PMC8240953.
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Tob Induc Dis. 2021 Jun 29;19:56. doi: 10.18332/tid/138336. eCollection 2021.

Changes in salivary proteome before and after cigarette smoking in smokers compared to sham smoking in nonsmokers: A pilot study.

Tobacco induced diseases

Indu Sinha, Jennifer Modesto, Nicolle M Krebs, Anne E Stanley, Vonn A Walter, John P Richie, Joshua E Muscat, Raghu Sinha

Affiliations

  1. Department of Biochemistry and Molecular Biology, Penn State Cancer Institute, Hershey, United States.
  2. Department of Public Health Sciences, Penn State Cancer Institute, Hershey, United States.
  3. Mass Spectrometry and Proteomics Core, Penn State University College of Medicine, Hershey, United States.

PMID: 34239408 PMCID: PMC8240953 DOI: 10.18332/tid/138336

Abstract

INTRODUCTION: Smoking is the leading cause of preventable disease. Although smoking results in an acute effect of relaxation and positive mood through dopamine release, smoking is thought to increase stress symptoms such as heart rate and blood pressure from nicotine-induced effects on the HPA axis and increased cortisol. Despite the importance in understanding the mechanisms in smoking maintenance, little is known about the overall protein and physiological response to smoking. There may be multiple functions involved that if identified might help in improving methods for behavioral and pharmacological interventions. Therefore, our goal for this pilot study was to identify proteins in the saliva that change in response to an acute smoking event versus acute sham smoking event in smokers and non-smokers, respectively.

METHODS: We employed the iTRAQ technique followed by Mass Spectrometry to identify differentially expressed proteins in saliva of smokers and non-smokers after smoking cigarettes and sham smoking, respectively. We also validated some of the salivary proteins by ELISA or western blotting. In addition, salivary cortisol and salivary amylase (sAA) activity were measured.

RESULTS: In all, 484 salivary proteins were identified. Several proteins were elevated as well as decreased in smokers compared to non-smokers. Among these were proteins associated with stress response including fibrinogen alpha, cystatin A and sAA. Our investigation also highlights methodological considerations in study design, sampling and iTRAQ analysis.

CONCLUSIONS: We suggest further investigation of other differentially expressed proteins in this study including ACBP, A2ML1, APOA4, BPIB1, BPIA2, CAH1, CAH6, CYTA, DSG1, EST1, GRP78, GSTO1, sAA, SAP, STAT, TCO1, and TGM3 that might assist in improving methods for behavioral and pharmacological interventions for smokers.

© 2021 Sinha I. et al.

Keywords: non-smokers; proteomics; saliva; salivary alpha amylase; smokers

Conflict of interest statement

The authors have each completed and submitted an ICMJE form for disclosure of potential conflicts of interest. The authors declare that they have no competing interests, financial or otherwise, relate

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