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Lodde V, Luciano AM, Musmeci G, et al. A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve. Animals (Basel). 2021;11(7)doi: 10.3390/ani11071936.
Lodde, V., Luciano, A. M., Musmeci, G., Miclea, I., Tessaro, I., Aru, M., Albertini, D. F., Franciosi, F., Van, D., & Sitkin, S. B. (2021). A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve. Animals : an open access journal from MDPI, 11(7), . https://doi.org/10.3390/ani11071936
Lodde, Valentina, et al. "A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve." Animals : an open access journal from MDPI vol. 11,7 (2021). doi: https://doi.org/10.3390/ani11071936
Lodde V, Luciano AM, Musmeci G, Miclea I, Tessaro I, Aru M, Albertini DF, Franciosi F, Van D, Sitkin SB. A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve. Animals (Basel). 2021 Jun 29;11(7). doi: 10.3390/ani11071936. PMID: 34209664; PMCID: PMC8300191.
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Animals (Basel). 2021 Jun 29;11(7). doi: 10.3390/ani11071936.

A Nuclear and Cytoplasmic Characterization of Bovine Oocytes Reveals That Cysteamine Partially Rescues the Embryo Development in a Model of Low Ovarian Reserve.

Animals : an open access journal from MDPI

Valentina Lodde, Alberto Maria Luciano, Giulia Musmeci, Ileana Miclea, Irene Tessaro, Mariella Aru, David F Albertini, Federica Franciosi, Van, Sitkin

Affiliations

  1. Reproductive and Developmental Biology Lab., Dipartimento di Scienze Veterinarie per la Salute la Produzione Animale e la Sicurezza Alimentare 'Carlo Cantoni', Università degli Studi di Milano, 20133 Milano, Italy.
  2. Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
  3. Bedford Research Foundation, Bedford, MA 01730, USA.

PMID: 34209664 PMCID: PMC8300191 DOI: 10.3390/ani11071936

Abstract

Decreased oocyte quality is a major determinant of age-associated fertility decline. Similarly, individuals affected by early ovarian aging carry low-quality oocytes. Using an established bovine model of early ovarian aging, we investigated key features of 'quality' oocyte maturation, associated with the onset of egg aneuploidy and reproductive aging, such as histone modifications, mitochondria distribution and activity, reduced glutathione (GSH) content, and gap junction functionality. Bovine ovaries were classified according to the antral follicle count (AFC), and the retrieved oocytes were processed immediately or matured in vitro. We observed alterations in several cellular processes, suggesting a multifactorial etiology of the reduced oocyte quality. Furthermore, we performed a rescue experiment for one of the parameters considered. By adding cysteamine to the maturation medium, we experimentally increased the free radical scavenger ability of the 'low competence' oocytes and obtained a higher embryo development. Our findings show that adopting culture conditions that counteract the free radicals has a positive impact on the quality of 'compromised' oocytes. Specifically, cysteamine treatment seems to be a promising option for treating aging-related deficiencies in embryo development.

Keywords: GSH; antral follicle count; cow; cysteamine; early ovarian aging; embryo development; gap junctions; histone modifications; mitochondria; oocyte quality

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