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Selcer K, Balasubramonian B, Miller D, et al. Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids. Steroids. 2021;174:108890doi: 10.1016/j.steroids.2021.108890.
Selcer, K., Balasubramonian, B., Miller, D., Kerr, J., DiFrancesco, M., Ojha, S., & Urbano, R. (2021). Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids. Steroids, 174108890. https://doi.org/10.1016/j.steroids.2021.108890
Selcer, Kyle, et al. "Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids." Steroids vol. 174 (2021): 108890. doi: https://doi.org/10.1016/j.steroids.2021.108890
Selcer K, Balasubramonian B, Miller D, Kerr J, DiFrancesco M, Ojha S, Urbano R. Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids. Steroids. 2021 Oct;174:108890. doi: 10.1016/j.steroids.2021.108890. Epub 2021 Jul 17. PMID: 34280393.
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Steroids. 2021 Oct;174:108890. doi: 10.1016/j.steroids.2021.108890. Epub 2021 Jul 17.

Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids.

Steroids

Kyle Selcer, Barathi Balasubramonian, Dylan Miller, Jade Kerr, Mia DiFrancesco, Sanjana Ojha, Rachel Urbano

Affiliations

  1. Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA. Electronic address: [email protected].
  2. Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

PMID: 34280393 DOI: 10.1016/j.steroids.2021.108890

Abstract

Steroid hormones often circulate in the blood as inactive sulfated forms, such as estrone sulfate and dehydroepiandrosterone sulfate. The enzyme steroid sulfatase (STS) converts these steroids into active forms, mainly estrogens, in peripheral tissues. We have previously characterized STS activity in human and mouse breast and bone tissues, and we have shown that STS can provide estrogens to these tissues from circulating sulfated precursors. This study was designed to characterize STS activity in a mouse fibroblast cell line (NIH-3T3). Using a radioactive estrone sulfate (E

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Cortisol; Estrogens; Estrone sulfate; Fibroblasts; Glucocorticoids; Mouse; NIH-3T3 cells; Regulation; Steroid sulfatase; Sulfated steroids

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